Antibody for human TdT

Terminal deoxynucleotidyl transferase (TdT) is a unique DNA polymerase, which, unlike the other ones, does not need a template for DNA synthesis, but instead it adds nucleotides randomly to the end of a single-stranded DNA chain.

This enzyme is essential for vertebrate adaptive immune system, as it helps to generate enormous quantities of different specific B cell receptor and T cell receptor variants. Its ability to elongate single-stranded DNA is also being used e.g. for identification of fragmented DNA in apoptotic cells, or for synthesis of oligonucleotides. However, TdT is also an important marker in the diagnosis of leukemias. As it is expressed only in certain phase of B cell and T cell development, when the rearrangement of immune receptor genes occurs, it can be used for distinguishing between premature and mature lymphoid cells, both in flow cytometry and immunohistochemistry. For example it is a marker in the diagnosis of acute lymphoblastic leukemia, and its distinguishing from Burkitt lymphoma and other lymphoid malignancies.

The mouse monoclonal antibody 41A, recognizing human TdT, is suitable for immunohistochemistry (paraffin sections), flow cytometry, Western blotting, and ELISA.

Currently available formate:
Anti-Hu TdT Purified

FIG. 1: Separation of REH cells stained with anti-human TdT (41A) purified antibody (concentration in the sample 0.9 μg/ml, GAM APC, red-filled) from REH cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).


Would you like to have our TdT antibody in other formates? Let us know!