The mouse monoclonal antibody TAp63-4.1, recognizing the transactivation isoform of p63 (TAp63), is suitable for immunohistochemistry (paraffin sections), immunocytochemistry, Western blotting, and immunoprecipitation. Immunohistochemistry of p63 has a clinical relevance for certain tumor types, but investigations have been hampered by a lack of well characterized antibodies that are specific for p63, do not cross-react with the related p53 and p73 proteins, and allow for discrimination between p63 isoforms TAp63 and deltaNp63 with opposite functional properties. The antibody TAP63-4.1 matches these requirements.
The mouse monoclonal antibody PANp63-6.1 recognizing both TAp63, and deltaNp63 form of p63 is suitable for for immunohistochemistry (paraffin sections), immunocytochemistry, Western blotting, and immunoprecipitation. Together with the above mentioned antibody TAp63-4.1, this clone provides an important tool for analysis of p63 isoforms, which has clinical relevance.
Fig. 1: Comparison of p63 isoforms: transactivation isoform TAp63, and deltaNp63 without transactivation domain (Signoretti et al., Am J Pathol. 2000, 157(6):1769-75).
Fig. 2: Western blotting analysis of p63 isoforms using mouse monoclonal antibody PANp63.1. This antibody detects both TAp63 and deltaNp63 isoforms, as demonstrated on particular transfectants.
Mouse monoclonal antibody DNp73-1.1 recognizing the isoform of p73, which lacks the N-terminal transactivation domain (deltaNp73), is suitable for immunocytochemistry, Western blotting, and immunoprecipitation. Similarly to TAp63, investigations of this p53 family member have been hampered by a lack of well characterized antibodies that are specific for p73 and do not cross-react with the related p53 and p63 proteins, as well as a lack of antibodies that specifically recognize either the TAp73 or deltaNp73 variants. The monoclonal antibody DNp73-1.1 helps to fill this gap.
Anti-Hu deltaNp73 Purified
Fig. 3: Western blotting analysis of p73 isoforms using mouse monoclonal antibody DNp73-1.1. This antibody is suitable for distinguishing between them. It detected specifically deltaNp73 isoform in H1299/deltaNp73 transfectants, whereas it ignored TAp73 isoform in H1299/TAp73 transfectants.
We present here a brief comparison of TB3, UCHT1, and MEM-57 anti-CD3 flow cytometric antibodies, that have been submitted to HLDA workshops in past.
The monoclonal antibody JOVI.1 recognizes the TRBC1+ region and enables fast and reliable assessment of clonal restriction of TCR C genes by flow cytometry.
Today we introduce two mouse monoclonal antibody clones and two rabbit polyclonal antibodies, that have been added to our portfolio: Anti-TIAR (clone 6E3), anti-human CD300b (clone B24), polyclonal anti-mCherry, and polyclonal anti-mPlum.