The mouse monoclonal antibody TAp63-4.1, recognizing the transactivation isoform of p63 (TAp63), is suitable for immunohistochemistry (paraffin sections), immunocytochemistry, Western blotting, and immunoprecipitation. Immunohistochemistry of p63 has a clinical relevance for certain tumor types, but investigations have been hampered by a lack of well characterized antibodies that are specific for p63, do not cross-react with the related p53 and p73 proteins, and allow for discrimination between p63 isoforms TAp63 and deltaNp63 with opposite functional properties. The antibody TAP63-4.1 matches these requirements.
The mouse monoclonal antibody PANp63-6.1 recognizing both TAp63, and deltaNp63 form of p63 is suitable for for immunohistochemistry (paraffin sections), immunocytochemistry, Western blotting, and immunoprecipitation. Together with the above mentioned antibody TAp63-4.1, this clone provides an important tool for analysis of p63 isoforms, which has clinical relevance.
Fig. 1: Comparison of p63 isoforms: transactivation isoform TAp63, and deltaNp63 without transactivation domain (Signoretti et al., Am J Pathol. 2000, 157(6):1769-75).
Fig. 2: Western blotting analysis of p63 isoforms using mouse monoclonal antibody PANp63.1. This antibody detects both TAp63 and deltaNp63 isoforms, as demonstrated on particular transfectants.
Mouse monoclonal antibody DNp73-1.1 recognizing the isoform of p73, which lacks the N-terminal transactivation domain (deltaNp73), is suitable for immunocytochemistry, Western blotting, and immunoprecipitation. Similarly to TAp63, investigations of this p53 family member have been hampered by a lack of well characterized antibodies that are specific for p73 and do not cross-react with the related p53 and p63 proteins, as well as a lack of antibodies that specifically recognize either the TAp73 or deltaNp73 variants. The monoclonal antibody DNp73-1.1 helps to fill this gap.
Anti-Hu deltaNp73 Purified
Fig. 3: Western blotting analysis of p73 isoforms using mouse monoclonal antibody DNp73-1.1. This antibody is suitable for distinguishing between them. It detected specifically deltaNp73 isoform in H1299/deltaNp73 transfectants, whereas it ignored TAp73 isoform in H1299/TAp73 transfectants.
Trop-2 is thought to be associated with the epithelial phenotype of cancer cells and many studies have reported that epithelial markers positively correlate with its expression, whereas mesenchymal markers typically exert negative correlation.
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. Among other applications, they also can be used as markers for detection and characterization of circulating tumor cells.