The mouse monoclonal antibody TAp63-4.1, recognizing the transactivation isoform of p63 (TAp63), is suitable for immunohistochemistry (paraffin sections), immunocytochemistry, Western blotting, and immunoprecipitation. Immunohistochemistry of p63 has a clinical relevance for certain tumor types, but investigations have been hampered by a lack of well characterized antibodies that are specific for p63, do not cross-react with the related p53 and p73 proteins, and allow for discrimination between p63 isoforms TAp63 and deltaNp63 with opposite functional properties. The antibody TAP63-4.1 matches these requirements.
The mouse monoclonal antibody PANp63-6.1 recognizing both TAp63, and deltaNp63 form of p63 is suitable for for immunohistochemistry (paraffin sections), immunocytochemistry, Western blotting, and immunoprecipitation. Together with the above mentioned antibody TAp63-4.1, this clone provides an important tool for analysis of p63 isoforms, which has clinical relevance.
Fig. 1: Comparison of p63 isoforms: transactivation isoform TAp63, and deltaNp63 without transactivation domain (Signoretti et al., Am J Pathol. 2000, 157(6):1769-75).
Fig. 2: Western blotting analysis of p63 isoforms using mouse monoclonal antibody PANp63.1. This antibody detects both TAp63 and deltaNp63 isoforms, as demonstrated on particular transfectants.
Mouse monoclonal antibody DNp73-1.1 recognizing the isoform of p73, which lacks the N-terminal transactivation domain (deltaNp73), is suitable for immunocytochemistry, Western blotting, and immunoprecipitation. Similarly to TAp63, investigations of this p53 family member have been hampered by a lack of well characterized antibodies that are specific for p73 and do not cross-react with the related p53 and p63 proteins, as well as a lack of antibodies that specifically recognize either the TAp73 or deltaNp73 variants. The monoclonal antibody DNp73-1.1 helps to fill this gap.
Anti-Hu deltaNp73 Purified
Fig. 3: Western blotting analysis of p73 isoforms using mouse monoclonal antibody DNp73-1.1. This antibody is suitable for distinguishing between them. It detected specifically deltaNp73 isoform in H1299/deltaNp73 transfectants, whereas it ignored TAp73 isoform in H1299/TAp73 transfectants.
The importance of HLA-G is documented by the fact that there are more than 2 500 papers on HLA-G and Google returns more than 7,5 million entries related to this molecule.
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