Select one of the colors we offer and let EXBIO conjugate the protein or antibody of your choice. We offer conjugation of any antibody from our catalog with a broad range of fluorochromes or other molecules according to your request. We can also custom conjugate your own antibodies using our successful conjugation processes:
Conjugation with low-molecular weight fluorochromes, biotin or horseradish peroxidase (HRP):
• Receipt of antibody or other protein from a customer.
• Dialysis of the antibody or other protein.
• Activation of HRP and transfer to the conjugation buffer by gel filtration (only CC006).
• Conjugation.
• Removal of unconjugated fluorochrome, biotin or HRP.
• Stabilization of the conjugate and final adjustment.
• QC tests: Spectrophotometric determination of antibody concentration and F/P ratio (F/P ratio not determined in case of biotin or HRP).
• Delivery of the conjugate to the customer.
Conjugation with high-molecular weight fluorochromes (phycobilliproteins or tandem dyes):
• Receipt of antibody or other protein from a customer.
• Activation of fluorochrome and the antibody or other protein.
• Transfer to the conjugation buffer by gel filtration.
• Conjugation.
• Purification of conjugate by size-exclusion chromatography (optimally conjugated fraction is isolated).
• Stabilization of the conjugate and final adjustment.
• QC tests: Spectrophotometric determination of antibody concentration.
• Delivery of the conjugate to the customer.
In case of your interest, please:
1/ fill the Custom Conjugation form attached below and send it by fax (+420 261 090 660) or by e-mail to us
2/ we will reply to you by e-mail and discuss with you all necessary details
3/ then we will ask you to send us an official Purchase Order and the antibody by some courier (UPS, FedEx, TNT...)
4/ we will confirm you acceptance of your Purchase Order and delivery of your antibody
5/ when your product is ready, we will contact you again and inform you about the despatch date of your product, courier tracking number and all other details.
For further details, please contact us by e-mail here...
The prices mentioned below correspond to conjugation of 1 - 5 mg of antibody (input amount). The yield of conjugate depends on antibody properties and type of conjugate.
Custom conjugation prices in EUR:
CC001Cat. No.: Service: Unit: Price: Conjugation with FITC 1 - 5 mg 220 EUR CC002 Conjugation with PE 1 - 5 mg 440 EUR CC003 Conjugation with APC 1 - 5 mg 440 EUR CC004 Conjugation with PerCP 1 - 5 mg 550 EUR CC005 Conjugation with Biotin 1 - 5 mg 220 EUR CC006 Conjugation with HRP 1 - 5 mg 440 EUR CC007 Conjugation with small organic molecule* 1 - 5 mg 220 EUR CC008 Conjugation with a tandem dye* 1 - 5 mg 550 EUR
* Customer may be required to supply commercialy available small organic fluorochrome to prepare the requested conjugate.
download files:
Custom conjugation - form D