APC

Laser: Red Diode/HeNe Laser
Excitation: 633 nm, 647 nm
Emission: 660 nm

APC
(Allophycocyanin), a large protein (105 kDa), emerging as one of the brightest fluorescent probes for FACS analysis. APC is photosynthetic pigment found in bluegreen algae and contains many fluors. Typically, only one APC molecule is conjugated to an antibody and direct APC conjugates are relatively easy to make. APC has almost perfect quantum efficiency and emission in a region of the spectrum with extremely low cellular autofluorescence (maximum approx. 660 nm). APC is one of the brightest dyes used today.