Recombinant Human Vimentin
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Expressed in E. coli. DNA sequence encoding human Vimentin (amino acid residues 1 – 466; Accession # X56134) was fused with a His tag at the N-terminus.
Human Vimentin (53.7 kDa) is class III intermediate filament protein, which belongs to the intermediate filament family. It is highly expressed in the cells of mesenchymal origin. Mesenchyme later differentiates into other types of structures and systems: blood cells, endothelial cells, smooth muscle cells, circulatory system, lymphatic system. Vimentin has been used as a specific mesenchymal marker in differential diagnosis of undifferentiated neoplasms. Vimentin is widely distributed in the cytoplasm and is phosphorylated by several protein kinases in vitro.
- Inhibition Assays
- Western Blotting
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
*Evans RM: Vimentin: the conundrum of the intermediate filament gene family. Bioessays 1998. 20(1): 79-86. [Abstract]
*Schweitzer SC, Evans RM: Vimentin and lipid metabolism. Subcell Biochem. 1998. 31: 437-62. [Abstract]
*Yoon M, Moir RD, Prahlad V, Goldman RD: Motile properties of vimentin intermediate filament networks in living cells. J Cell Biol. 1998. 143(1): 147-57. [Abstract] [Full Text]
*Norlén L, Masich S, Goldie KN, Hoenger A: Structural analysis of vimentin and keratin intermediate filaments by cryoelectron tomography. Exp. Cell Res. 2007. 313(10): 2217-27. [Abstract]
For research use only. Not for drug, diagnostic or other use.
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