System Certification ISO 9001:2000
Exbio Antibodies CZ / SK eShop
a-z list

Recombinant human TRAIL-R1 (DR4/ TNFRSF10A)/Fc Chimera

Technical Information Request    Inquiry for Bulk Prices

Product No. Form Quantity Stock Price Datasheet Shop
  EXB0006 Lyophilized 0.05 mg yes choose region PDF datasheetHTML datasheet buy
- In stock! Ready for immediate despatch - Manufacture on order

Source

Produced in HEK 293 cells. The extracellular domain of human TRAIL-R1 (aa 1 - 238) is fused to the Fc portion of human IgG1.

Description

DR4 is a transmembrane protein, also called TRAIL receptor 1 (TRAIL-R1). The ligand for this DR4 death receptor has been identified and termed TRAIL, which is a member of the TNF family. DR4, as many other receptors (Fas, TNFR1, etc.), mediates apoptosis and NF kappaB activation in many cells and tissues.
Apoptosis, a programmed cell death, is a operating process in normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by coupled of certain cytokines (TNF family - TNF, Fas ligand) and their death domain containing receptors (TNFR1, Fas receptor).

Regulatory Status

Database Links


Applications:

  • Loading control
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

References

  • Unpublished.
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse Monoclonal to CD261 / TRAIL-R1 DR-4-02 (IgG1)
  • Example Data


    DR4 SDS
    Fig. 1. SDS-PAGE


    Fig.1. SDS-PAGE Electrophoresis of TRAIL-R1 (DR4) / Fc recombinant protein (12% separating gel; reducing conditions).
    Lane 1 to 3: TRAIL-R1 (DR4)/ Fc recombinant protein (2000 ng, 1000 ng, 500 ng per lane). Staining with Coomassie Blue.








    DR4
    Fig. 2. Western Blotting


    Fig.2. Western Blotting of TRAIL-R1 (DR4) / Fc recombinant protein. Reducing conditions.
    Lane 1 to 5: Immunostaining with TRAIL-R1 (DR4) specific antibody DR-4-02 (Cat. No. 10-403-C100; dilution 2 μg/ml ). Detection with Goat anti-mouse / HRP secondary antibody. Amount of TRAIL-R1 (DR4)/ Fc recombinant protein loaded on each lane: (1) 370 ng, (2) 150 ng, (3) 75 ng, (4) 37 ng, (5) 15 ng.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
    © 2003 EXBIO. All rights reserved.
    terms and conditions | privacy policy