Recombinant human PAG / Cbp
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Expressed in E. coli. DNA sequence encoding intracellular domain of human Cbp/ PAG (amino acids 41- 432) was fused with a His tag at the N-terminus.
Csk binding protein, also known as PAG (Phosphoprotein Associated with Glycosphingolipid-enriched microdomains) is a ubiquitously expressed transmembrane adaptor protein present in membrane rafts.
Cbp/PAG has a pivotal role in the assembly of negative regulators in these membrane domains.
Cbp/PAG consists of a short extracellular domain (16 to 18 residues), a transmembrane domain (20 residues), and a large cytoplasmic domain (387 to 396 residues); it contains ten tyrosine residues, nine of which are potential substrates for Src kinases, and two proline-rich regions.
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
Product Specific References
*Brdicka T, Pavlistova D, Leo A, Bruyns E, Korinek V, Angelisova P, Scherer J, Shevchenko A, Hilgert I, Cerny J, Drbal K, Kuramitsu Y, Kornacker B, Horejsi V, Schraven B. Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase csk and is involved in regulation of T cell activation. J Exp Med. 2000 May 1;191(9):1591-604.
For research use only. Not for drug, diagnostic or other use.
Rabbit Polyclonal to PAG / Cbp
Mouse Monoclonal to PAG / Cbp PAG-C1 (IgG2b)
Mouse Monoclonal to PAG / Cbp MEM-255 (IgG2a)
Fig. 1. SDS-PAGE
Fig.1. SDS-PAGE Electrophoresis of Cbp / PAG recombinant protein (cat. no. EXB0009, previously RL-004-C100). 12% separating gel, 6% stacking gel; reducing conditions.
Lane 1-5: Cbp / PAG recombinant protein (2000 ng, 1000 ng, 500 ng, 200 ng, 100 ng per lane). Staining with Coomassie Blue.
Fig. 2. Western Blotting
Fig.2. Western Blotting analysis of Cbp / PAG recombinant protein (cat. no. EXB0009, previously RL-004-C100). Reducing conditions.
Lane 1-4: immunostaining with anti-Cbp rabbit polyclonal antibody (EXBIO cat. no. 11-409-C100; dilution 0.5 μg/ml). Detection with Goat anti-rabbit / HRP secondary antibody. Amount of Cbp/PAG recombinant protein loaded on each lane: (1) 100 ng, (2) 50 ng, (3) 20 ng, (4) 10 ng.