Native human Prostate-specific antigen / PSA
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EXB0011 |
Lyophilized |
0.1 mg |
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- In stock! Ready for immediate despatch
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Source
Prostate-specific antigen (PSA) was purified from human seminal plasma by ion-exchange chromatography and gel filtration. The purified antigen was dialyzed against d.H2O and lyophilized.
Description
Prostate-specific antigen (PSA) is a glycoprotein of approx. 30 kDa found mainly in prostatic tissue and seminal fluid; it is produced by normal prostate cells. The molecule consists of approximately 92% protein and 8% carbohydrate. This enzyme participates in the dissolution of the seminal fluid coagulum and plays an important role in fertility. The highest amounts of PSA are found in the seminal fluid; some PSA escapes the prostate and can be found in the serum. This serum component has been used to track the response to therapy in men with prostate cancer.
Database Links
Applications:
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
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General references
*Zhang WM, Leinonen J, Kalkkinen N, Dowell B, Stenman UH: Purification and characterization of different molecular forms of prostate-specific antigen in human seminal fluid. Clin Chem. 1995 Nov;41(11):1567-73.
[Abstract]
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For research use only. Not for drug, diagnostic or other use.
Related Products
Mouse Monoclonal to Prostate-specific antigen (PSA) A67-B/E3 (IgG1)
Mouse Monoclonal to Prostate-specific antigen (PSA) A67-B/E3 (IgG1)
Example Data
Fig. 1. Western Blotting
Fig.1. Western Blotting analysis of native human Prostate-specific antigen (cat. no. EXB0011; previously NL-008-C100). Reducing conditions.
Lane 1-3: immunostaining of PSA antigen (1 mg/ml) with anti-human Prostate-specific antigen antibody (A67-B/E3; EXBIO cat. no. 11-289-C100; dilution 1 μg/ml (1), 0.5 μg/ml (2), 0.2 μg/ml (3)). Detection by Goat anti-mouse / HRP secondary antibody.
