Expressed in E. coli. DNA sequence encoding human Vimentin (amino acid residues 1 – 466; Accession # X56134) was fused with a His tag at the N-terminus.
Prostate-specific antigen (PSA) was purified from human seminal plasma by ion-exchange chromatography and gel filtration. The purified antigen was dialyzed against d.H2O and lyophilized.