System Certification ISO 9001:2000
Exbio Antibodies CZ / SK eShop
by categories | a-z list | Application type: Flow Cytometry | Immunohistochemistry | Functional Studies
Antigen Clone Research area Protocol
Acidic Cytokeratins AE1    
Actin HHF35   Application note:Antigen retrieval steps generally not required, but e.g. in case of arterial smooth muscle cells or myoepithelial cells, pepsin or trypsin pretreatment is recommended.
AGR2+AGR3 AGR3.4   Recommended dilution:5 μg/ml
Positive tissue:human colon
AGR3 AGR3.1   Recommended dilution: 1 μg/ml
Positive tissue: breast cancer
Albumin AL-01   Recommended dilution: 5 μg/ml
Positive tissue: liver
alpha-Fetoprotein AFP-11   Recommended dilution:10 μg/ml
Positive tissue: hepatocellular carcinoma
Application note:heat mediated antigen retrieval (sodium citrate buffer); mAb incubation 1 hour / RT; detection DAB
alpha-tubulin TU-16   Recommended dilution: 10 μg/ml
alpha-tubulin TU-01   Recommended dilution: 5 μg/ml
Positive tissue: heart
Basic Cytokeratins AE3    
Bcl2 Bcl-2/100    
beta Endorphin B31.15   Recommended dilution: 10 - 20 μg/ml
Staining technique: Standard ABC technique (DAB+)
Pretreatment: Heat retrieval (microwave oven)
Incubation: overnight at 4C
Positive tissue: Human pituitary gland
betaIII-tubulin TU-20   Recommended dilution: 10 μg/ml
Staining technique: Standard ABC technique (DAB+)
Pretreatment: 0.1% pepsin (trypsin) in 0.1 M HCl; incubation 30 min in RT; or High temperature citrate buffer antigen retrieval Positive tissue: neuronal tissue
beta-tubulin TU-06   Recommended dilution: 5 μg/ml
Positive tissue: heart
beta2-Microglobulin B2M-01   Recommended dilution: 10 μg/ml
Positive tissue: kidney glomeruli
beta2-Microglobulin B2M-02   Recommended dilution: 5 μg/ml
Positive tissue: liver
Blood Group A HE-193    
Blood Group ABH HE-10   The antibody HE-10 is excellent as a tumour marker in patients of blood group B and 0.
Blood Group B HEB-29    
Blood Group Lewis a 7LE   Recommended dilution:5 μg/ml
Positive control:colorectal adenocarcinoma
Application note:heat mediated antigen retrieval (sodium citrate); mAb incubation 1 hour / RT; detection DAB
Blood Group Lewis b 2-25LE   Recommended dilution:5-10 μg/ml
Positive control:colorectal adenocarcinoma
Application note:heat mediated antigen retrieval (sodium citrate); mAb incubation 1 hour / RT; detection DAB
Cdk1 POH-1   Recommended dilution:10 μg/ml
Positive tissue: thymus
CD1b SN13    
CD107a B-T47    
CD107a H4A3    
CD107b H4B4    
CD120a H398    
CD134 Ber-ACT35    
CD138 MI15    
CD14 B-A8   Recommended dilution: 2-10 μg/ml
Positive tissue: PML brain sections
Application note:
Positive staining on human PML brain sections was mainly observed on monocytes in the lumenal side of brain blood vessels, and on some perivascular cells adjacent to medium-sized vessels.
Heat retrieval of antigen is recommeded.
CD146 P1H12    
CD147 MEM-M6/1   Recommended dilution: 10 μg/ml
Positive tissue: testis
CD147 MEM-M6/2   Recommended dilution: 10 μg/ml
Positive tissue: testis
CD15 MMA    
CD162 TB5   Positive tissue:tonsil, spleen
CD18 MEM-48   Recommended dilution: 10 μg/ml
Positive tissue: spleen, microglia
CD18 (mouse) M18/2    
CD184 / CXCR4 12G5    
CD20 MEM-269    
CD200 OX-104    
CD235a JC159   Recommended dilution:10 μg/ml
CD243 UIC2   Application note: heat mediated antigen retrieval in citrate buffer pH 6
CD271 NGFR5   Recommended dilution:1-2 μg/ml
Positive tissue: melanoma
Application note: heat-mediated antigen retrieval
CD30 MEM-268   Recommended dilution: 5 μg/ml
Positive tissue: thymus
CD30 Ber-H8    
CD326 / EpCAM 323/A3 Tumour Markers Recommended dilution: 1-10 μg/ml
Pretreatment: Tissue section digestion with pepsin (15 min at RT or 10 min at 37C, 1 mg / ml Tris-HCl, pH 2.0).
Positive tissue: Breast carcinoma
CD326 / EpCAM VU-1D9   Recommended dilution: 5-10 μg/ml
Positive control: colon epithelium
Application note: antigen retrieval: heat (sodium citrate) + trypsin; mAb incubation: 1 hour / RT; detection DAB
CD34 QBEnd-10   Recommended dilution: 1-2 μg/ml
CD34 4H11[APG]   Recommended dilution: 10 μg/ml
Positive tissue: placenta endothelium
CD35 E11   Application note:heat mediated antigen retrieval
CD38 HIT2   Recommended dilution:10 μg/ml
CD41 (mouse) MWReg30    
CD43 MEM-59   Positive tissue:spleen, thymus, tonsil
Recommended dilution:10 μg/ml
CD44 MEM-263   Recommended dilution: 10 μg/ml
Positive tissue: uterus, myometrium
CD44 IM7   Positive tissue:mouse spleen
CD45 MEM-28   No pre-treatment of tissue sections is essential.
CD45 HI30    
CD45RA MEM-56   No pre-treatment of tissue sections is essential.
CD45RB MEM-55   No pre-treatment of tissue sections is essential.
CD45RB MEM-143   Recommended dilution:10 μg/ml
Positive control:human tonsil
CD45R (human, mouse) RA3-6B2    
CD45R0 UCHL1   Pretreatment: This product does not require protein digestion pretreatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Positive tissue: tonsil
CD48 MEM-102    
CD5 MEM-32   Recommended dilution: 20 μg/ml
Positive tissue: spleen
CD5 (mouse) 53-7.3    
CD50 MEM-04   Recommended dilution:10 μg/ml
Positive tissue: tonsil
CD52 HI186    
CD54 MEM-111   Recommended dilution: 10 μg/ml
Positive tissue: thymus, RE cells
CD54 1H4   Recommended dilution: 10 μg/ml
Application Note: Prolonged fixation in buffered formalin can destroy the epitope. Hight temperature antigen unmasking technique is required
CD55 MEM-118   Recommended dilution: 10 μg/ml
CD56 MEM-188   Recommended dilution: 1:50
Pretreatment: Heat retrieval (microwave oven) in citrate buffer, standard ABC visualization.
Positive tissue: Positive membrane-specific reaction in adjacent peripheral nerve
Staining of paraprostatic autonomic ganglion.
Staining technique: Standard ABC technique (DAB+).
CD57 TB01    
CD59 MEM-43   Recommended dilution: 10 μg/ml
Positive tissue: placenta
CD59 MEM-43/5   Recommended dilution: 5 μg/ml
CD6 MEM-98   Recommended dilution: 10 μg/ml
Positive tissue: spleen
CD62L / L-Selectin (mouse) MEL-14    
CD63 MEM-259   Recommended dilution:10 μg/ml
Positive tissue: spleen
CD64 10.1   Application note:There can occur problems with paraformaldehyde fixation
CD66acde CLB-gran/10   Recommended dilution:20 μg/ml
Positive tissue:human colon
CD66e CB30 Tumour Markers Staining technique: Standard ABC technique (DAB+)
Recommended dilution: 10 μg/ml (1:100)
Positive tissue: Adenocarcinoma of colon
Pretreatment: 0,1% pepsin in 0,1 M HCl for 30 min at room temperature
CD7 MEM-186   Recommended dilution: 5 μg/ml
Positive tissue: spleen
CD79a HM57   Recommended dilution:10 μg/ml
CD79a HM47    
CD8a (mouse) 53-6.7    
CD8a (rat) OX-8    
CD81 M38    
CD82 C33    
CD88 / C5aR S5/1    
CD9 MEM-61   Recommended dilution: 20 μg/ml
Positive tissue: prostate
CD95 / Fas LT95   Positive tissue:tonsil
CD98 MEM-108   Recommended dilution: 10 μg/ml
Positive tissue: liver
CD99R MEM-131   Recommended dilution: 10 μg/ml
Positive tissue: thymic lymphocytes
Clusterin Hs-3   Recommended dilution: 10 μg/ml
Positive tissue: Sertoli cells
c-Myc 9E10   Recommended dilution: 5-10 μg/ml
Positive tissue: perfused brain sections, liver, spleen
C-peptide of Proinsulin C-PEP-01   Recommended dilution:
purified 2-5 μg/ml
biotin 25 μg/ml
Positive control:
human pancreas (islets of Langerhans)
Cyclin D1 CD1.1   Pretreatment: Heat treatment, sodium citrate buffer pH 6.0.
Cyclin D1 DCS-6    
Cytokeratin (Pan-reactive) C-11    
Cytokeratin 10 VIK-10   Recommended dilution: 10 μg/ml
Positive tissue: skin
Pretreatment: trypsin/temperature 37 C
Cytokeratin 10+13 DE-K13 Intermediate Filaments: Cytokeratins Staining technique: Standard ABC technique (DAB+).
Recommended dilution: 10 μg/ml (1:100)
Positive tissue: tonsil
Pretreatment: 0.1% pepsin in 0.1 M HCl for 30 min at room temperature; or Target Retrieval Solution - High pH
Application note: The antibody DE-K13 reacts exclusively with Cytokeratin 13.
Cytokeratin 18 DC-10 Intermediate Filaments: Cytokeratins Recommended dilution: 10 μg/ml
Positive tissue: colon, skin
Cytokeratin 18 DA-7 Intermediate Filaments: Cytokeratins Recommended dilution: 10 μg/ml
Positive tissue: colon, skin
Cytokeratin 18 C-04 Intermediate Filaments: Cytokeratins Recommended dilution:
10 μg/ml
Positive control: colon, kidney
Cytokeratin 19 BA-17    
Cytokeratin 19 A53-B/A2    
Cytokeratin 5+18 C-50   Recommended dilution:10 μg/ml
Positive control:Human prostate
Cytokeratin 7+17 C-46   Recommended dilution: 20 μg/ml
Pretreatment: 0,1% pepsin, 30 min, 37C
Incubation: 4C overnight
Positive tissue: pancreas
Application note: chromogen DAB
Cytokeratin 8 C-43   Recommended dilution: 20 μg/ml
Positive tissue: prostate
Cytokeratin 8 C-51    
Fyn FYN-01   Recommended dilution: 5 μg/ml
Positive tissue: brain
Gamma-tubulin complex component 2 GCP2-01    
GCPII / PSMA GCP-04    
GFAP GF-02    
GFAP GA-5   Recommended dilution: 1-2 μg/ml
GFAP GF-01   Recommended dilution:10 μg/ml
Positive tissue: human brain (cortex, cerebellum)
The antibody GF-01 strongly stains astrocytes in human brain tissue sections but it is essentially negative on mouse and rat tissues.
Growth hormone (hGH) GH-45    
Growth hormone + HRP GH-Px-2    
HLA-DR L243    
HLA-DR+DP MEM-136    
HLA-DR+DP HL-38    
HLA-DR+DP HL-40    
HLA-E MEM-E/06   Recommended dilution:10 μg/ml
Positive tissue: spleen
HLA-E MEM-E/02   Recommended dilution: 5-10 μg/ml
Positive control:Human placenta
Application note:Heat antigen retrieval (sodium citrate); incubation with mAb: 1 hour / RT; detection DAB
HLA-E MEM-E/07   Recommended dilution:10 μg/ml
Positive tissue: spleen
HLA-G MEM-G/2    
HLA-G 2A12    
HLA-G 4H84   Recommended dilution:10 μg/ml
HLA-G MEM-G/1   Recommended dilution: 1:60 to 1:100
Incubation: 1 h at RT
Positive tissue: Human placenta - extravillous cytotrophoblast
Staining technique: Standard ABC technique
Pretreatment: Heat retrieval in 0.01M citrate buffer (4x2 min. in microwave oven)
HLA-G 5A6G7   Recommended dilution: 10 μg/ml
Positive tissue: placenta tissue
IFN-gamma 4S.B3    
IgA AD3   Recommended dilution:10 μg/ml
Positive tissue: colon
IgA secretory component SC-05    
Insulin IN-05    
Kappa light chains A8B5    
Kappa light chains MEM-09    
Lambda light chains 4C2    
LIME LIME-10   Recommended dilution: 10 μg/ml
LIME Polyclonal    
Ly6G (mouse) RB6-8C5    
MAP2ab MT-07   Recommended dilution: 10 μg/ml
Positive tissue: brain
MAP2ab MT-08   Recommended dilution: 10 μg/ml
Positive tissue: brain
MFG MFG-06   Application note: Strongly positive on human epithelia-based structures.
MHC Class II (mouse) M5/114    
Mouse IgG1 Isotype Control MOPC-21    
Mouse IgG2b Isotype Control MPC-11    
Neurofilament heavy protein NF-01    
Neurofilament medium protein NF-09    
NHERF1 / EBP50 EBP-10   Recommended dilution: 5 μg/ml
Nitrotyrosine EM-30   Recommended dilution:10 μg/ml
Positive tissue:colon cancer epithelium, prostate hyperplasia
NTAL / LAB NAP-03   Recommended dilution: 5 μg/ml
Positive tissue: tonsil, germinal center
NTAL / LAB Polyclonal   Recommended dilution:10 μg/ml
Positive tissue: spleen
PAG / Cbp PAG-C1   Positive tissue:Appendix (germinal center of lymphatic follicle
Application note:heat-mediated antigen retrieval in citrate buffer pH 6.1
PAG / Cbp Polyclonal   Positive tissue:tonsil, colon germinal center
PAG / Cbp MEM-255   Positive tissue:tonsil, spleen
PCNA PC10    
Perforin dG9    
Placental alkaline phosphatase H7E8   Recommended dilution: 10 μg/ml
Prostate-specific antigen (PSA) A67-B/E3    
p21Waf1 WA-1   Positive tissue: colon carcinoma
p53 BP53-12    
p53 (Phospho-Ser392) FP3.2 [FPS392]   Staining technique: standard ABC technique (DAB+)
Pretreatment: high temperature antigen retrieval (microwave, pressure cooker) in 10 mM citrate buffer pH 6.0 or 1 mM EDTA-NaOH buffer pH 8.0
Recommended dilution: 10 μg/ml
Incubation:1 hour at RT; or overnight at 4C
Positive tissue: breast carcinoma with high level of wild-type p53
Sialyl Lewis a 121SLE   Recommended dilution:10 μg/ml
Positive control:colorectal adenocarcinoma
Application note:heat mediated antigen retrieval (sodium citrate); mAb incubation 1 hour / RT; detection DAB
SLP76 Polyclonal   Recommended dilution:10 μg/ml
Positive tissue: thymus
SLP76 SLP-76/03   Recommended dilution: 10 μg/ml
Positive tissue: thymus
STAT1 SM1   Recommended dilution: 5 μg/ml
Positive tissue: lung macrophages
STIM1 CDN3H4   Recommended dilution: 5 μg/ml
Syk SYK-01   Recommended dilution: 5 μg/ml
Positive tissue: tonsil B cells
TCF4 / TF7L2 Polyclonal   Recommended dilution:10 μg/ml
Positive tissue: colon
TCR alpha/beta (rat) R73    
TCR beta (mouse) H57-597    
TCR gamma/delta B1    
Tenascin T2H5   Application note:Immunohistochemical detection of tenascin is valuable for studies of tissue differentiation and tumour growth. The antibody T2H5 is excellent for staining of paraffin-embedded tissue sections.
Thyroglobulin 2H11   Recommended dilution: 1:80 - 1:100
Pretreatment: Heat retrieval in 0.01M citrate buffer, pH 6 (microwave oven)
Incubation: 1h at RT
Positive tissue: thyroid gland, follicular adenoma
Transferrin HTF-14   Recommended dilution: 10 μg/ml
Positive tissue: placenta
Vimentin VI-10   Recommended dilution: 5 μg/ml
Positive tissue: skin fibroblast
5-bromodeoxyuridine (BrdU) Bu20a    
5-bromodeoxyuridine (BrdU) MoBu-1   Application note: excellent

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