| Antigen | Clone | Research area | Protocol |
|---|---|---|---|
| Actin | HHF35 | Application note:Antigen retrieval steps generally not required, but e.g. in case of arterial smooth muscle cells or myoepithelial cells, pepsin or trypsin pretreatment is recommended. |
|
| AGR2+AGR3 | AGR3.4 | Recommended dilution:5 μg/ml Positive tissue:human colon |
|
| AGR3 | AGR3.1 | Recommended dilution: 1 μg/ml Positive tissue: breast cancer |
|
| Albumin | AL-01 | Recommended dilution: 5 μg/ml Positive tissue: liver |
|
| alpha-Fetoprotein | AFP-11 | Recommended dilution:10 μg/ml Positive tissue: hepatocellular carcinoma Application note:heat mediated antigen retrieval (sodium citrate buffer); mAb incubation 1 hour / RT; detection DAB |
|
| alpha-tubulin | TU-01 | Recommended dilution: 5 μg/ml Positive tissue: heart |
|
| alpha-tubulin | TU-16 | Recommended dilution: 10 μg/ml |
|
| Bcl2 | Bcl2/100 | ||
| beta Endorphin | B31.15 | Recommended dilution: 10 - 20 μg/ml Staining technique: Standard ABC technique (DAB+) Pretreatment: Heat retrieval (microwave oven) Incubation: overnight at 4°C Positive tissue: Human pituitary gland |
|
| betaIII-tubulin | TU-20 | Recommended dilution: 10 μg/ml Staining technique: Standard ABC technique (DAB+) Pretreatment: 0.1% pepsin (trypsin) in 0.1 M HCl; incubation 30 min in RT; or High temperature citrate buffer antigen retrieval Positive tissue: neuronal tissue |
|
| beta-tubulin | TU-06 | Recommended dilution: 5 μg/ml Positive tissue: heart |
|
| beta2-microglobulin | B2M-01 | Recommended dilution: 10 μg/ml Positive tissue: kidney glomeruli |
|
| beta2-microglobulin | B2M-02 | Recommended dilution: 5 μg/ml Positive tissue: liver |
|
| Blood Group A | HE-193 | ||
| Blood Group ABH | HE-10 | The antibody HE-10 is excellent as a tumour marker in patients of blood group B and 0. | |
| Blood Group B | HEB-29 | ||
| Blood Group Lewis a | 7LE | Recommended dilution:5 μg/ml Positive control:colorectal adenocarcinoma Application note:heat mediated antigen retrieval (sodium citrate); mAb incubation 1 hour / RT; detection DAB |
|
| Blood Group Lewis b | 2-25LE | Recommended dilution:5-10 μg/ml Positive control:colorectal adenocarcinoma Application note:heat mediated antigen retrieval (sodium citrate); mAb incubation 1 hour / RT; detection DAB |
|
| Cdk1 | POH-1 | Recommended dilution:10 μg/ml Positive tissue: thymus |
|
| CD107a | B-T47 | ||
| CD107a | H4A3 | ||
| CD107b | H4B4 | ||
| CD14 | B-A8 | Recommended dilution: 2-10 μg/ml Positive tissue: PML brain sections Application note: Positive staining on human PML brain sections was mainly observed on monocytes in the lumenal side of brain blood vessels, and on some perivascular cells adjacent to medium-sized vessels. Heat retrieval of antigen is recommeded. |
|
| CD147 | MEM-M6/1 | Recommended dilution: 10 μg/ml Positive tissue: testis |
|
| CD147 | MEM-M6/2 | Recommended dilution: 10 μg/ml Positive tissue: testis |
|
| CD162 | TB5 | Positive tissue:tonsil, spleen |
|
| CD18 | MEM-48 | Recommended dilution: 10 μg/ml Positive tissue: spleen, microglia |
|
| CD18 (mouse) | M18/2 | ||
| CD20 | MEM-269 | ||
| CD271 | NGFR5 | Recommended dilution:1-2 μg/ml Positive tissue: melanoma Application note: heat-mediated antigen retrieval |
|
| CD30 | MEM-268 | Recommended dilution: 5 μg/ml Positive tissue: thymus |
|
| CD326 / EpCAM | 323/A3 | Tumour Markers | Recommended dilution: 1-10 μg/ml Pretreatment: Tissue section digestion with pepsin (15 min at RT or 10 min at 37°C, 1 mg / ml Tris-HCl, pH 2.0). Positive tissue: Breast carcinoma |
| CD326 / EpCAM | VU-1D9 | Recommended dilution: 5-10 μg/ml Positive control: colon epithelium Application note: antigen retrieval: heat (sodium citrate) + trypsin; mAb incubation: 1 hour / RT; detection DAB |
|
| CD34 | 4H11[APG] | Recommended dilution: 10 μg/ml Positive tissue: placenta endothelium |
|
| CD34 | QBEnd-10 | Recommended dilution: 1-2 μg/ml |
|
| CD340 / ErbB2 / HER2 | Polyclonal | Recommended dilution: 10 μg/ml in PBS with 10% FCS and 0.1 % NaN3 Staining technique: standard ABC technique (DAB+) Pretreatment: Fill the staining dishes with 10 mM citrate buffer pH 6.0 (pretreatment solution). Put the staining dishes with the pretreatment solution in the water bath and heat on 95-99°C. Sink the deparaffin sections into the preheated pretreatment solution. Once again heat the water bath and pretreatment solution with sections on 95-99°C - incubate for 40 min in 95-99°C. Remove the dishes with sections from the water bath and keep them cool down for 20 min in RT. Rinse the sections 3 x 5 min in washing buffer (PBS). Incubation: overnight at 4°C Positive tissue: breast carcinoma Application note: Warning: The sections must not drain!! |
|
| CD35 | E11 | Application note:heat mediated antigen retrieval |
|
| CD38 | HIT2 | Recommended dilution:10 μg/ml |
|
| CD43 | MEM-59 | Positive tissue:spleen, thymus, tonsil Recommended dilution:10 μg/ml |
|
| CD44 | MEM-263 | Recommended dilution: 10 μg/ml Positive tissue: uterus, myometrium |
|
| CD44 | IM7 | Positive tissue:mouse spleen |
|
| CD45 | MEM-28 | No pre-treatment of tissue sections is essential. | |
| CD45 | HI30 | ||
| CD45RA | MEM-56 | No pre-treatment of tissue sections is essential. | |
| CD45RB | MEM-55 | No pre-treatment of tissue sections is essential. | |
| CD45RB | MEM-143 | Recommended dilution:10 μg/ml Positive control:human tonsil |
|
| CD45R0 | UCHL1 | Pretreatment: This product does not require protein digestion pretreatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Positive tissue: tonsil |
|
| CD48 | MEM-102 | ||
| CD5 | MEM-32 | Recommended dilution: 20 μg/ml Positive tissue: spleen |
|
| CD50 | MEM-04 | Recommended dilution:10 μg/ml Positive tissue: tonsil |
|
| CD52 | HI186 | ||
| CD54 | 1H4 | Recommended dilution: 10 μg/ml Application Note: Prolonged fixation in buffered formalin can destroy the epitope. Hight temperature antigen unmasking technique is required |
|
| CD54 | MEM-111 | Recommended dilution: 10 μg/ml Positive tissue: thymus, RE cells |
|
| CD55 | MEM-118 | Recommended dilution: 10 μg/ml |
|
| CD56 | MEM-188 | Recommended dilution: 1:50 Pretreatment: Heat retrieval (microwave oven) in citrate buffer, standard ABC visualization. Positive tissue: Positive membrane-specific reaction in adjacent peripheral nerve Staining of paraprostatic autonomic ganglion. Staining technique: Standard ABC technique (DAB+). |
|
| CD59 | MEM-43 | Recommended dilution: 10 μg/ml Positive tissue: placenta |
|
| CD59 | MEM-43/5 | Recommended dilution: 5 μg/ml |
|
| CD6 | MEM-98 | Recommended dilution: 10 μg/ml Positive tissue: spleen |
|
| CD62L / L-Selectin (mouse) | MEL-14 | ||
| CD63 | MEM-259 | Recommended dilution:10 μg/ml Positive tissue: spleen |
|
| CD64 | 10.1 | Application note:There can occur problems with paraformaldehyde fixation | |
| CD66acde | CLB-gran/10 | Recommended dilution:20 μg/ml Positive tissue:human colon |
|
| CD66e | CB30 | Tumour Markers | Staining technique: Standard ABC technique (DAB+) Recommended dilution: 10 μg/ml (1:100) Positive tissue: Adenocarcinoma of colon Pretreatment: 0,1% pepsin in 0,1 M HCl for 30 min at room temperature |
| CD7 | MEM-186 | Recommended dilution: 5 μg/ml Positive tissue: spleen |
|
| CD79a | HM57 | Recommended dilution:10 μg/ml |
|
| CD8a (mouse) | 53-6.7 | ||
| CD8a (rat) | OX-8 | ||
| CD81 | M38 | ||
| CD82 | C33 | ||
| CD9 | MEM-61 | Recommended dilution: 20 μg/ml Positive tissue: prostate |
|
| CD95 / Fas | LT95 | Positive tissue:tonsil |
|
| CD98 | MEM-108 | Recommended dilution: 10 μg/ml Positive tissue: liver |
|
| CD99R | MEM-131 | Recommended dilution: 10 μg/ml Positive tissue: thymic lymphocytes |
|
| Clusterin | Hs-3 | Recommended dilution: 10 μg/ml Positive tissue: Sertoli cells |
|
| c-Myc | 9E10 | Recommended dilution: 5-10 μg/ml Positive tissue: perfused brain sections, liver, spleen |
|
| C-peptide of Proinsulin | C-PEP-01 | Recommended dilution: purified 2-5 μg/ml biotin 25 μg/ml Positive control: human pancreas (islets of Langerhans) |
|
| Cyclin D1 | CD1.1 | Pretreatment: Heat treatment, sodium citrate buffer pH 6.0. |
|
| Cytokeratin (Pan-reactive) | C-11 | ||
| Cytokeratin 10 | VIK-10 | Recommended dilution: 10 μg/ml Positive tissue: skin Pretreatment: trypsin/temperature 37 °C |
|
| Cytokeratin 10+13 | DE-K13 | Intermediate Filaments: Cytokeratins | Staining technique: Standard ABC technique (DAB+). Recommended dilution: 10 μg/ml (1:100) Positive tissue: tonsil Pretreatment: 0.1% pepsin in 0.1 M HCl for 30 min at room temperature; or Target Retrieval Solution - High pH Application note: The antibody DE-K13 reacts exclusively with Cytokeratin 13. |
| Cytokeratin 18 | DC-10 | Intermediate Filaments: Cytokeratins | Recommended dilution: 10 μg/ml Positive tissue: colon, skin |
| Cytokeratin 18 | DA-7 | Intermediate Filaments: Cytokeratins | Recommended dilution: 10 μg/ml Positive tissue: colon, skin |
| Cytokeratin 18 | C-04 | Intermediate Filaments: Cytokeratins | Recommended dilution: 10 μg/ml Positive control: colon, kidney |
| Cytokeratin 19 | A53-B/A2 | ||
| Cytokeratin 19 | BA-17 | ||
| Cytokeratin 5+18 | C-50 | Recommended dilution:10 μg/ml Positive control:Human prostate |
|
| Cytokeratin 7+17 | C-46 | Recommended dilution: 20 μg/ml Pretreatment: 0,1% pepsin, 30 min, 37°C Incubation: 4°C overnight Positive tissue: pancreas Application note: chromogen DAB |
|
| Cytokeratin 8 | C-43 | Recommended dilution: 20 μg/ml Positive tissue: prostate |
|
| Cytokeratin 8 | C-51 | ||
| Fyn | FYN-01 | Recommended dilution: 5 μg/ml Positive tissue: brain |
|
| GCPII / PSMA | GCP-04 | ||
| GFAP | GF-02 | ||
| GFAP | GA-5 | Recommended dilution: 1-2 μg/ml | |
| GFAP | GF-01 | Recommended dilution:10 μg/ml Positive tissue: human brain (cortex, cerebellum) The antibody GF-01 strongly stains astrocytes in human brain tissue sections but it is essentially negative on mouse and rat tissues. |
|
| Growth hormone (hGH) | GH-45 | ||
| Growth hormone + HRP | GH-Px-2 | ||
| HLA-DR | L243 | ||
| HLA-E | MEM-E/02 | Recommended dilution: 5-10 μg/ml Positive control:Human placenta Application note:Heat antigen retrieval (sodium citrate); incubation with mAb: 1 hour / RT; detection DAB |
|
| HLA-E | MEM-E/07 | Recommended dilution:10 μg/ml Positive tissue: spleen |
|
| HLA-E | MEM-E/06 | Recommended dilution:10 μg/ml Positive tissue: spleen |
|
| HLA-G | 5A6G7 | Recommended dilution: 10 μg/ml Positive tissue: placenta tissue |
|
| HLA-G | MEM-G/2 | ||
| HLA-G | MEM-G/1 | Recommended dilution: 1:60 to 1:100 Incubation: 1 h at RT Positive tissue: Human placenta - extravillous cytotrophoblast Staining technique: Standard ABC technique Pretreatment: Heat retrieval in 0.01M citrate buffer (4x2 min. in microwave oven) |
|
| HLA-G | 2A12 | ||
| HLA-G | 4H84 | Recommended dilution:10 μg/ml |
|
| IgA | AD3 | Recommended dilution:10 μg/ml Positive tissue: colon |
|
| IgA secretory component | SC-05 | ||
| Insulin | IN-05 | ||
| Kappa light chains | MEM-09 | ||
| Kappa light chains | A8B5 | ||
| Lambda light chains | 4C2 | ||
| LIME | Polyclonal | ||
| LIME | LIME-10 | Recommended dilution: 10 μg/ml |
|
| Ly6G (mouse) | RB6-8C5 | ||
| MAP2ab | MT-07 | Recommended dilution: 10 μg/ml Positive tissue: brain |
|
| MAP2ab | MT-08 | Recommended dilution: 10 μg/ml Positive tissue: brain |
|
| MFG | MFG-06 | Application note: Strongly positive on human epithelia-based structures. | |
| MHC Class II (mouse) | M5/114 | ||
| Mouse IgG1 Isotype Control | MOPC-21 | ||
| Mouse IgG2b Isotype Control | MPC-11 | ||
| Neurofilament heavy protein | NF-01 | ||
| Neurofilament medium protein | NF-09 | ||
| NHERF1 / EBP50 | EBP-10 | Recommended dilution: 5 μg/ml |
|
| Nitrotyrosine | EM-30 | Recommended dilution:10 μg/ml Positive tissue:colon cancer epithelium, prostate hyperplasia |
|
| NTAL / LAB | NAP-03 | Recommended dilution: 5 μg/ml Positive tissue: tonsil, germinal center |
|
| NTAL / LAB | Polyclonal | Recommended dilution:10 μg/ml Positive tissue: spleen |
|
| PAG / Cbp | MEM-255 | Positive tissue:tonsil, spleen |
|
| PAG / Cbp | Polyclonal | Positive tissue:tonsil, colon germinal center |
|
| PAG / Cbp | PAG-C1 | Positive tissue:Appendix (germinal center of lymphatic follicle Application note:heat-mediated antigen retrieval in citrate buffer pH 6.1 |
|
| Placental alkaline phosphatase | H7E8 | Recommended dilution: 10 μg/ml |
|
| Prostate-specific antigen (PSA) | A67-B/E3 | ||
| p21Waf1 | WA-1 | Positive tissue: colon carcinoma |
|
| p53 | BP53-12 | ||
| p53 (Phospho-Ser392) | FP3.2 [FPS392] | Staining technique: standard ABC technique (DAB+) Pretreatment: high temperature antigen retrieval (microwave, pressure cooker) in 10 mM citrate buffer pH 6.0 or 1 mM EDTA-NaOH buffer pH 8.0 Recommended dilution: 10 μg/ml Incubation:1 hour at RT; or overnight at 4°C Positive tissue: breast carcinoma with high level of wild-type p53 |
|
| Sialyl Lewis a | 121SLE | Recommended dilution:10 μg/ml Positive control:colorectal adenocarcinoma Application note:heat mediated antigen retrieval (sodium citrate); mAb incubation 1 hour / RT; detection DAB |
|
| SLP76 | SLP-76/03 | Recommended dilution: 10 μg/ml Positive tissue: thymus |
|
| SLP76 | Polyclonal | Recommended dilution:10 μg/ml Positive tissue: thymus |
|
| STAT1 | SM1 | Recommended dilution: 5 μg/ml Positive tissue: lung macrophages |
|
| STIM1 | CDN3H4 | Recommended dilution: 5 μg/ml |
|
| Syk | SYK-01 | Recommended dilution: 5 μg/ml Positive tissue: tonsil B cells |
|
| TCF4 / TF7L2 | Polyclonal | Recommended dilution:10 μg/ml Positive tissue: colon |
|
| TCR alpha/beta (rat) | R73 | ||
| TCR beta (mouse) | H57-597 | ||
| Tenascin | T2H5 | Application note:Immunohistochemical detection of tenascin is valuable for studies of tissue differentiation and tumour growth. The antibody T2H5 is excellent for staining of paraffin-embedded tissue sections. | |
| Thyroglobulin | 2H11 | Recommended dilution: 1:80 - 1:100 Pretreatment: Heat retrieval in 0.01M citrate buffer, pH 6 (microwave oven) Incubation: 1h at RT Positive tissue: thyroid gland, follicular adenoma |
|
| Transferrin | HTF-14 | Recommended dilution: 10 μg/ml Positive tissue: placenta |
|
| Vimentin | VI-10 | Recommended dilution: 5 μg/ml Positive tissue: skin fibroblast |
|
| 5-bromodeoxyuridine (BrdU) | Bu20a | ||
| 5-bromodeoxyuridine (BrdU) | MoBu-1 | Application note: excellent |