System Certification ISO 9001:2000
Exbio Antibodies CZ / SK eShop
by categories | a-z list | Application type: Flow Cytometry | Immunohistochemistry | Functional Studies
Antigen Clone Research area Protocol
5-bromodeoxyuridine (BrdU) MoBu-1   Application note: excellent
AGR3 AGR3.1   Recommended dilution: 1 μg/ml
Positive tissue: breast cancer
Actin HHF35   Application note:Antigen retrieval steps generally not required, but e.g. in case of arterial smooth muscle cells or myoepithelial cells, pepsin or trypsin pretreatment is recommended.
Albumin AL-01   Recommended dilution: 5 μg/ml
Positive tissue: liver
Blood Group A HE-193    
Blood Group ABH HE-10   The antibody HE-10 is excellent as a tumour marker in patients of blood group B and 0.
Blood Group B HEB-29    
Blood Group Lewis a 7LE   Recommended dilution:5 μg/ml
Positive control:colorectal adenocarcinoma
Application note:heat mediated antigen retrieval (sodium citrate); mAb incubation 1 hour / RT; detection DAB
Blood Group Lewis b 2-25LE   Recommended dilution:5 μg/ml
Positive control:colorectal adenocarcinoma
Application note:heat mediated antigen retrieval (sodium citrate); mAb incubation 1 hour / RT; detection DAB
C-peptide of Proinsulin C-PEP-01   Recommended dilution:
purified 2-5 μg/ml
biotin 25 μg/ml
Positive control:
human pancreas (islets of Langerhans)
CD14 B-A8   Recommended dilution: 2-10 μg/ml
Positive tissue: PML brain sections
Application note:
Positive staining on human PML brain sections was mainly observed on monocytes in the lumenal side of brain blood vessels, and on some perivascular cells adjacent to medium-sized vessels.
Heat retrieval of antigen is recommeded.
CD147 MEM-M6/2   Recommended dilution: 10 μg/ml
Positive tissue: testis
CD147 MEM-M6/1   Recommended dilution: 10 μg/ml
Positive tissue: testis
CD162 TB5   Positive tissue:tonsil, spleen
CD18 MEM-48   Recommended dilution: 10 μg/ml
Positive tissue: spleen, microglia
CD20 MEM-269  
CD30 MEM-268   Recommended dilution: 5 μg/ml
Positive tissue: thymus
CD326 / EpCAM VU-1D9   Recommended dilution: 5-10 μg/ml
Positive control: colon epithelium
Application note: antigen retrieval: heat (sodium citrate) + trypsin; mAb incubation: 1 hour / RT; detection DAB
CD326 / EpCAM 323/A3 Tumour Markers Recommended dilution: 1-10 μg/ml
Pretreatment: Tissue section digestion with pepsin (15 min at RT or 10 min at 37°C, 1 mg / ml Tris-HCl, pH 2.0).
Positive tissue: Breast carcinoma
CD34 4H11[APG]   Recommended dilution: 10 μg/ml
Positive tissue: placenta endothelium
CD34 QBEnd-10   Recommended dilution: 1-2 μg/ml
CD340 / ErbB2 / HER2 Polyclonal   Recommended dilution: 10 μg/ml in PBS with 10% FCS and 0.1 % NaN3
Staining technique: standard ABC technique (DAB+)
Pretreatment: Fill the staining dishes with 10 mM citrate buffer pH 6.0 (pretreatment solution). Put the staining dishes with the pretreatment solution in the water bath and heat on 95-99°C. Sink the deparaffin sections into the preheated pretreatment solution. Once again heat the water bath and pretreatment solution with sections on 95-99°C - incubate for 40 min in 95-99°C. Remove the dishes with sections from the water bath and keep them cool down for 20 min in RT. Rinse the sections 3 x 5 min in washing buffer (PBS).
Incubation: overnight at 4°C
Positive tissue: breast carcinoma
Application note: Warning: The sections must not drain!!
CD43 MEM-59   Positive tissue:thymus, tonsil
CD44 MEM-263   Recommended dilution: 10 μg/ml
Positive tissue: uterus, myometrium
CD45 MEM-28   No pre-treatment of tissue sections is essential.
CD45R0 UCHL1   Pretreatment: This product does not require protein digestion pretreatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Positive tissue: tonsil
CD45RA MEM-56   No pre-treatment of tissue sections is essential.
CD45RB MEM-55   No pre-treatment of tissue sections is essential.
CD48 MEM-102    
CD5 MEM-32   Recommended dilution: 20 μg/ml
Positive tissue: spleen
CD52 HI186    
CD54 1H4   Recommended dilution: 10-20 μg/ml
Application Note: Prolonged fixation in buffered formalin can destroy the epitope. Hight temperature antigen unmasking technique is required
CD54 MEM-111   Recommended dilution: 10 μg/ml
Positive tissue: thymus, RE cells
CD55 MEM-118   Recommended dilution: 10 μg/ml
CD56 MEM-188   Recommended dilution: 1:50
Pretreatment: Heat retrieval (microwave oven) in citrate buffer standard ABC visualization.
Positive tissue: Positive membrane-specific reaction in adjacent peripheral nerve
Staining of paraprostatic autonomic ganglion.
Staining technique: Standard ABC technique (DAB+).
CD59 MEM-43   Recommended dilution: 10 μg/ml
Positive tissue: placenta
CD59 MEM-43/5   Recommended dilution: 5 μg/ml
CD6 MEM-98   Recommended dilution: 10 μg/ml
Positive tissue: spleen
CD63 MEM-259   Recommended dilution:10 μg/ml
Positive tissue: spleen
CD66e CB30 Tumour Markers Staining technique: Standard ABC technique (DAB+)
Recommended dilution: 10 μg/ml (1:100)
Positive tissue: Adenocarcinoma of colon
Pretreatment: 0,1% pepsin in 0,1 M HCl for 30 min at room temperature
CD7 MEM-186   Recommended dilution: 5 μg/ml
Positive tissue: spleen
CD74 LN2   Recommended dilution: 10 μg/ml
CD79a HM57    
CD81 M38    
CD8a (mouse) 53-6.7  
CD9 MEM-61   Recommended dilution: 20 μg/ml
Positive tissue: prostate
CD95 / Fas LT95   Positive tissue:tonsil
CD98 MEM-108   Recommended dilution: 10 μg/ml
Positive tissue: liver
CD99R MEM-131   Recommended dilution: 10 μg/ml
Positive tissue: thymic lymphocytes
Cdk1 POH-1    
Clusterin Hs-3   Recommended dilution: 10 μg/ml
Positive tissue: Sertoli cells
Cyclin D1 CD1.1   Pretreatment: Heat treatment, sodium citrate buffer pH 6.0.
Cytokeratin (Pan-reactive) C-11    
Cytokeratin 10 VIK-10   Recommended dilution: 10 μg/ml
Positive tissue: skin
Pretreatment: trypsin/temperature 37 °C
Cytokeratin 10+13 DE-K13 Intermediate Filaments: Cytokeratins Staining technique: Standard ABC technique (DAB+).
Recommended dilution: 10 μg/ml (1:100)
Positive tissue: tonsil
Pretreatment: 0.1% pepsin in 0.1 M HCl for 30 min at room temperature; or Target Retrieval Solution - High pH
Application note: The antibody DE-K13 reacts exclusively with Cytokeratin 13.
Cytokeratin 18 C-04 Intermediate Filaments: Cytokeratins  
Cytokeratin 18 DA-7 Intermediate Filaments: Cytokeratins Recommended dilution: 1:10 - 1:100
Positive tissue: epithelial tissue (skin)
Cytokeratin 18 DC-10 Intermediate Filaments: Cytokeratins Recommended dilution:1:10 - 1:100
Positive tissue: epithelial tissue (skin)
Cytokeratin 19 BA-17    
Cytokeratin 19 A53-B/A2    
Cytokeratin 5+18 C-50    
Cytokeratin 7+17 C-46   Recommended dilution: 20 μg/ml
Pretreatment: 0,1% pepsin, 30 min, 37°C
Incubation: 4°C overnight
Positive tissue: pancreas
Application note: chromogen DAB
Cytokeratin 8 C-51    
Cytokeratin 8 C-43   Recommended dilution: 20 μg/ml
Positive tissue: prostate
Fyn FYN-01   Recommended dilution: 5 μg/ml
Positive tissue: brain
GCPII / PSMA GCP-04    
GFAP GF-01   The antibody GF-01 strongly stains astrocytes in human brain tissue sections but it is essentially negative on mouse and rat tissues.
GFAP GF-02    
GFAP GA-5   Recommended dilution: 1-2 μg/ml
Growth hormone (hGH) GH-45    
Growth hormone + HRP GH-Px-2    
HLA-E MEM-E/02   Recommended dilution: 5-10 μg/ml
Positive control:Human placenta
Application note:Heat antigen retrieval (sodium citrate); incubation with mAb: 1 hour / RT; detection DAB
HLA-G MEM-G/2    
HLA-G MEM-G/1   Recommended dilution: 1:60 to 1:100
Incubation: 1 h at RT
Positive tissue: Human placenta - extravillous cytotrophoblast
Staining technique: Standard ABC technique
Pretreatment: Heat retrieval in 0.01M citrate buffer (4x2 min. in microwave oven)
HLA-G 5A6G7   Recommended dilution: 10 - 20 μg/ml
Positive tissue: placenta tissue
HLA-G 4H84    
HLA-G 2A12    
Insulin IN-05    
Kappa light chains A8B5    
Kappa light chains MEM-09    
LIME LIME-10   Recommended dilution: 10 μg/ml
Lambda light chains 4C2    
Ly6G (mouse) RB6-8C5    
MAP2ab MT-08   Recommended dilution: 10 μg/ml
Positive tissue: brain
MAP2ab MT-07   Recommended dilution: 10 μg/ml
Positive tissue: brain
MFG MFG-06   Application note: Strongly positive on human epithelia-based structures.
NHERF1 / EBP50 EBP-10   Recommended dilution: 5 μg/ml
NTAL / LAB NAP-03   Recommended dilution: 5 μg/ml
Positive tissue: tonsil, germinal center
Neurofilament heavy protein NF-01    
Neurofilament medium protein NF-09    
PAG / Cbp Polyclonal   Positive tissue:tonsil, colon germinal center
PAG / Cbp PAG-C1   Positive tissue:Appendix (germinal center of lymphatic follicle
Application note:heat-mediated antigen retrieval in citrate buffer pH 6.1
PAG / Cbp MEM-255   Positive tissue:tonsil, spleen
Placental alkaline phosphatase H7E8   Recommended dilution: 10 μg/ml
Prostate-specific antigen (PSA) A67-B/E3    
SLP76 SLP-76/03   Recommended dilution: 10 μg/ml
Positive tissue: thymus
STAT1 SM1   Recommended dilution: 5 μg/ml
Positive tissue: lung macrophages
STIM1 CDN3H4   Recommended dilution: 5 μg/ml
Secretory component SC-05    
Sialyl Lewis a 121SLE   Recommended dilution:10 μg/ml
Positive control:colorectal adenocarcinoma
Application note:heat mediated antigen retrieval (sodium citrate); mAb incubation 1 hour / RT; detection DAB
Syk SYK-01   Recommended dilution: 5 μg/ml
Positive tissue: tonsil B cells
TCR beta (mouse) H57-597    
Tenascin T2H5   Application note:Immunohistochemical detection of tenascin is valuable for studies of tissue differentiation and tumour growth. The antibody T2H5 is excellent for staining of paraffin-embedded tissue sections.
Thyroglobulin 2H11   Recommended dilution: 1:80 - 1:100
Pretreatment: Heat retrieval in 0.01M citrate buffer, pH 6 (microwave oven)
Incubation: 1h at RT
Positive tissue: thyroid gland, follicular adenoma
Transferrin HTF-14   Recommended dilution: 10 μg/ml
Positive tissue: placenta
Vimentin VI-10   Recommended dilution: 5 μg/ml
Positive tissue: skin fibroblast
alpha-Fetoprotein AFP-11   Recommended dilution:10 μg/ml
Positive tissue: hepatocellular carcinoma
Application note:heat mediated antigen retrieval (sodium citrate buffer); mAb incubation 1 hour / RT; detection DAB
alpha-tubulin TU-16   Recommended dilution: 10 μg/ml
alpha-tubulin TU-01   Recommended dilution: 5 μg/ml
Positive tissue: heart
beta Endorphin B31.15   Recommended dilution: 10 - 20 μg/ml
Staining technique: Standard ABC technique (DAB+)
Pretreatment: Heat retrieval (microwave oven)
Incubation: overnight at 4°C
Positive tissue: Human pituitary gland
beta-tubulin TU-06   Recommended dilution: 5 μg/ml
Positive tissue: heart
beta2-microglobulin B2M-01   Recommended dilution: 10 μg/ml
Positive tissue: kidney glomeruli
beta2-microglobulin B2M-02   Recommended dilution: 5 μg/ml
Positive tissue: liver
betaIII-tubulin TU-20   Recommended dilution: 10 μg/ml
Staining technique: Standard ABC technique (DAB+)
Pretreatment: 0.1% pepsin (trypsin) in 0.1 M HCl; incubation 30 min in RT; or High temperature citrate buffer antigen retrieval Positive tissue: neuronal tissue
c-Myc 9E10   Recommended dilution: 5-10 μg/ml
Positive tissue: perfused brain sections, liver, spleen
p21Waf1 WA-1   Positive tissue: colon carcinoma
p53 BP53-12    
p53 (phospho Ser392) FP3.2 [FPS392]   Staining technique: standard ABC technique (DAB+)
Pretreatment: high temperature antigen retrieval (microwave, pressure cooker) in 10 mM citrate buffer pH 6.0 or 1 mM EDTA-NaOH buffer pH 8.0
Recommended dilution: 10 μg/ml
Incubation:1 hour at RT; or overnight at 4°C
Positive tissue: breast carcinoma with high level of wild-type p53

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