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Mouse Monoclonal to HLA-F

3D11 (IgG1)

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Background

HLA-F, an MHC class I molecule, is a type I transmembrane protein (heavy chain), which forms heterodimers with beta-2 microglobulin (light chain) and binds to KIR3DS1, KIR3DS4, KIR3DL2, ILT2, ILT4, and TAP. Unlike most other HLA proteins, HLA-F is mainly localized in the endoplasmic reticulum and Golgi apparatus, with only a small amount present on the cell surface in some cell types; surface expression can be induced by cell activation. It is thought to bind a restricted subset of peptides for immune presentation. Multiple transcript variants encoding different isoforms have been found for HLA-F gene. These variants lack a coding exon found in transcripts from other HLA paralogues due to an altered splice acceptor site, resulting in a shorter cytoplasmic domain.

Specificity

The mouse monoclonal antibody 3D11 recognizes HLA-F, a 42 kDa type I transbembrane protein expressed on B cells, NK cells, monocytes, and T cells, but mainly in the endoplasmic reticulum and Golgi apparatus, only a small amount on the cell surface, where, however, it can be expressed after cell activation.

Regulatory Status

Immunogen

Inclusion body-derived HLA-F heavy chain

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
  • Western Blotting
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Lee N, Geraghty DE: HLA-F surface expression on B cell and monocyte cell lines is partially independent from tapasin and completely independent from TAP. J Immunol. 2003 Nov 15;171(10):5264-71. [Abstract] [Full Text]
  • *Burian A, Wang KL, Finton KA, Lee N, Ishitani A, Strong RK, Geraghty DE: HLA-F and MHC-I Open Conformers Bind Natural Killer Cell Ig-Like Receptor KIR3DS1. PLoS One. 2016 Sep 20;11(9):e0163297. [Abstract] [Full Text]
  • *Goodridge JP, Burian A, Lee N, Geraghty DE: HLA-F and MHC class I open conformers are ligands for NK cell Ig-like receptors. J Immunol. 2013 Oct 1;191(7):3553-62. [Abstract] [Full Text]
  • *Lee N, Ishitani A, Geraghty DE: HLA-F is a surface marker on activated lymphocytes. Eur J Immunol. 2010 Aug;40(8):2308-18. [Abstract]
  • *Geraghty D, Lee N, Wang KL, Ishitani A: HLA-F and receptor function on B cells and monocytes in the inflammatory response. J. Immunol. 2017, May 1, 198 (1 Supplement) 195.17.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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