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Mouse Monoclonal to CD133/2

293C3 (IgG2b)

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Background

CD133 (prominin 1) is a 5-transmembrane glycoprotein with extracellular N- and intracellular C-terminus. CD133 function remains to be elucidated, but it can be used as a cancer stem cell marker. Its expression pattern in progenitor cells is similar to CD34, i.e. on hematopoietic stem cells in bone marrow, cord blood, neural stem cells, retinoblastoma, or endothelial precursor cells (not mature endothelial cells). It is being used for identification and isolation of hematopoietic stem cells, including isolation for stem cell transplantation. Expression of CD133 correlates with differentiation of human colon cancer cells.

Specificity

The mouse monoclonal antibody 293C3 recognizes a glycosylated epitope 2 on human CD133, a 120 kDa glycoprotein of prominin family, expressed e.g. on progenitor cells. This antibody is important for identification of human renal progenitors.
HLDA VII Workshop

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
  • Western Blotting
  • Immunocytochemistry
    Application note:formaldehyde fixation can be used
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Richardson GD, Robson CN, Lang SH, Neal DE, Maitland NJ, Collins AT: CD133, a novel marker for human prostatic epithelial stem cells. J Cell Sci. 2004 Jul 15;117(Pt 16):3539-45. [Abstract] [Full Text]
  • *Lardon J, Corbeil D, Huttner WB, Ling Z, Bouwens L: Stem cell marker prominin-1/AC133 is expressed in duct cells of the adult human pancreas. Pancreas. 2008 Jan;36(1):e1-6. doi: 10.1097/mpa.0b013e318149f2dc. [Abstract]
  • *Barrantes-Freer A, Renovanz M, Eich M, Braukmann A, Sprang B, Spirin P, Pardo LA, Giese A, Kim EL: CD133 Expression Is Not Synonymous to Immunoreactivity for AC133 and Fluctuates throughout the Cell Cycle in Glioma Stem-Like Cells. PLoS One. 2015 Jun 18;10(6):e0130519. [Abstract] [Full Text]
  • *Zhang M, Liu Y, Feng H, Bian X, Zhao W, Yang Z, Gu B, Li Z, Liu Y: CD133 affects the invasive ability of HCT116 cells by regulating TIMP-2. Am J Pathol. 2013 Feb;182(2):565-76. [Abstract]
  • *Feng HL, Liu YQ, Yang LJ, Bian XC, Yang ZL, Gu B, Zhang H, Wang CJ, Su XL, Zhao XM: Expression of CD133 correlates with differentiation of human colon cancer cells. Cancer Biol Ther. 2010 Feb;9(3):216-23. [Abstract]
  • *Angelotti ML, Lazzeri E, Lasagni L, Romagnani P: Only anti-CD133 antibodies recognizing the CD133/1 or the CD133/2 epitopes can identify human renal progenitors. Kidney Int. 2010 Sep;78(6):620-1 [Abstract]
  • *Hermansen SK, Christensen KG, Jensen SS, Kristensen BW: Inconsistent immunohistochemical expression patterns of four different CD133 antibody clones in glioblastoma. J Histochem Cytochem. 2011 Apr;59(4):391-407. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

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