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Mouse Monoclonal to TPX2

TPX2-01 (IgG1)

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  11-818-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

TPX2 is a microtubule-associated protein, which is a critical regulator of mitosis. At the beginning of mitosis, TPX2 is released and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division. After completion of mitosis the TPX2 protein disappears, but has also role in DNA damage response. Its overexpression has been demonstrated in many types of carcinomas. TPX2 belongs to the markers of worse tumor prognosis. On the other hand, down-regulation of TPX2 can inhibit cancer cell proliferation, migration and invasion.

Specificity

The mouse monoclonal antibody TPX2-01 recognizes the epitope EPFVPKKEKKS (aa 636-646 in human) of TPX2, a microtubule-associated critical regulator of mitosis, which is overexpressed in many types of tumors and is a marker of worse prognosis in various cancers.

Regulatory Status

Immunogen

Human TPX2

Species Reactivity:

  • Human
  • Mouse
  • Rat

Negative Species:

Applications:

  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry (paraffin sections)
  • Immunocytochemistry
  • ELISA
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Miwa T, Kokuryo T, Yokoyama Y, Yamaguchi J, Nagino M: Therapeutic potential of targeting protein for Xklp2 silencing for pancreatic cancer. Cancer Med. 2015 Jul;4(7):1091-100 [Abstract] [Full Text]
  • *Zhang H, Zhang T, You Z, Zhang Y: Positive Surgical Margin, HPV Persistence, and Expression of Both TPX2 and PD-L1 Are Associated with Persistence/Recurrence of Cervical Intraepithelial Neoplasia after Cervical Conization. PLoS One. 2015 Dec 1;10(12):e0142868. [Abstract] [Full Text]
  • Wang J, Liu Z, Dou C, Han S, Li C, Tu K, Yang W: miR-491 inhibits the proliferation, invasion and migration of hepatocellular carcinoma cell via down-regulating TPX2 expression. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2016 Apr;32(4):512-7. [Abstract]
  • *Tomii C, Inokuchi M, Takagi Y, Ishikawa T, Otsuki S, Uetake H, Kojima K, Kawano T: TPX2 expression is associated with poor survival in gastric cancer. World J Surg Oncol. 2017 Jan 9;15(1):14. doi: 10.1186/s12957-016-1095-y [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    TPX2-01 WB


    Fig. 1. Western blotting analysis of TPX2 using monoclonal antibody TPX2-01 in A) human cell lines, B) murine (P19, NIH 3T3, Neuro-2a) and rat (RBL-2H3, L6) cell lines.


    TPX2-01 IP


    Fig. 2. Immunoprecipitation of TPX2 from nuclear extract of HEK293 cells using monoclonal antibody TPX2-01. 1) nuclear extract, 2) antibody, 3) immunoprecipitate, 4) carrier


    TPX2-01 ICC-IHC


    Fig. 3. Immunocytochemistry of TPX2 using monoclonal antibody TPX2-01 in glioblastoma cell line T98G: a) interphase, b) mitosis. c) Immunohistochemistry of TPX2 in U87MG human glioblastoma cells (orthotopic xenograft in murine brain). Bar: 20 μm.

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