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Mouse Monoclonal to R-Ras2 / TC21

EM-50 (IgG1)

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R-Ras2 / TC21 is the only member of R-Ras family of small GTPases that shows transforming activities similar to H-Ras, N-Ras, and K-Ras, and it is also structurally similar to them. R-Ras2 seems to play an important role in activating signal transduction pathways that control cell proliferation. Its mutations are associated with the growth of certain tumors, but also overexpression of the wild type form of R-Ras2 has been frequently detected in various carcinomas. Pseudogenes of R-Ras2 gene are found on chromosomes 1 and 2. Alternate splicing results in multiple transcript variants.


The mouse monoclonal antibody EM-50 recognizes R-Ras2 / TC21 protein and does not react with R-Ras1, H-Ras, K-Ras, and N-Ras.

Regulatory Status


Human TC21

Species Reactivity:

  • Human

Negative Species:


  • Flow Cytometry
  • Western Blotting
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • Larive RM, Moriggi G, Menacho-Márquez M, Cañamero M, de Álava E, Alarcón B, Dosil M, Bustelo XR: Contribution of the R-Ras2 GTP-binding protein to primary breast tumorigenesis and late-stage metastatic disease. Nat Commun. 2014 May 14;5:3881. [Abstract]
  • Gutierrez-Erlandsson S, Herrero-Vidal P, Fernandez-Alfara M, Hernandez-Garcia S, Gonzalo-Flores S, Mudarra-Rubio A, Fresno M, Cubelos B: R-RAS2 overexpression in tumors of the human central nervous system. Mol Cancer. 2013 Oct 23;12(1):127. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    EM-50 purified

    Fig. 1. Colocalization of R-Ras2/TC21 signal (red) and GFP signal (green) in COS cells transfected with R-Ras2/TC21-GFP construct, after fixation and permeabilization. R-Ras2/TC21 was detected using monoclonal antibody EM-50, purified.

    EM-50 PE

    Fig. 2. Intracellular staining of R-Ras2/TC21 in HEK-293 R-Ras2 transfectants using monoclonal antibody EM-50, PE.

    EM-50 purified

    Fig. 3. Intracellular staining of R-Ras2/TC21 in HEK-293 R-Ras2-GFP transfectants using monoclonal antibody EM-50, purified / GAM-APC.

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