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Mouse Monoclonal to PCNA

PC10 (IgG2a)

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Background

PCNA (proliferating cell nuclear antigen), which is the DNA polymerase delta auxiliary protein acting in homotrimeric form to increase the processivity of leading strand synthesis during DNA replication. PCNA is expressed in the nucleus of all proliferating cells. In response to DNA damage, it is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. PCNA is a useful marker of DNA synthesis, as its form not involved in DNA synthesis degradates in histological preparations in the presence of organic solvents.

Specificity

The mouse monoclonal antibody PC10 (also known as 3F81) recognizes PCNA, a 36 kDa conserved nuclear protein serving as a cofactor for DNA synthesis.

Immunogen

recombinant rat PCNA

Species Reactivity:

  • Human
  • Non-Human Primates
  • Mouse
  • Rat
  • Chicken
  • Drosophila

Negative Species:

Applications:

  • Flow Cytometry
    Application note: intracellular staining
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry (paraffin sections)
  • Immunohistochemistry (frozen sections)
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Landberg G, Roos G: Antibodies to proliferating cell nuclear antigen as S-phase probes in flow cytometric cell cycle analysis. Cancer Res. 1991 Sep 1;51(17):4570-4. [Abstract] [Full Text]
  • *Malkas LH, Herbert BS, Abdel-Aziz W, Dobrolecki LE, Liu Y, Agarwal B, Hoelz D, Badve S, Schnaper L, Arnold RJ, Mechref Y, Novotny MV, Loehrer P, Goulet RJ, Hickey RJ: A cancer-associated PCNA expressed in breast cancer has implications as a potential biomarker. Proc Natl Acad Sci U S A. 2006 Dec 19;103(51):19472-7. [Abstract] [Full Text]
  • *Dahm K, Hübscher U: Colocalization of human Rad17 and PCNA in late S phase of the cell cycle upon replication block. Oncogene. 2002 Oct 31;21(50):7710-9. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    PC10 pur


    Fig. 1. Intracellular staining of PCNA in K562 cells with anti-PCNA (PC10) purified, GAM-APC.


    PC10 PE


    Fig. 2. Intracellular staining of PCNA in K562 cells with anti-PCNA (PC10) PE.

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