categories: CD and Related Antigens (Human)

Mouse Monoclonal to CD273 / PD-L2

24F.10C12 (IgG2a)

Product No.	Form	Quantity	Price
11-178-C025	purified	0.025 mg	choose region
11-178-C100	purified	0.1 mg	choose region
1P-178-T025	PE	25 tests	choose region
1P-178-T100	PE	100 tests	choose region
1A-178-T100	APC	100 tests	choose region
A6-178-T100	Alexa Fluor® 647	100 tests	choose region

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Background

CD273 / PD-L2 (programmed death ligand-1), also known as B7-DC, is a member of the B7 family of regulatory proteins. It costimulates the proliferation of T cells, and mediates IFN gamma production. Ligation of CD273 on dendritic cells enhances dendritic cell activation and T cell responses. When interacting with CD279, it can act as a coinhibitor of the T cell function. CD273 expression is a useful marker to distinguish primary mediastinal B cell lymphoma from other diffuse large B cell lymphomas.

Entrez Gene: 9p24.2

Specificity

The mouse monoclonal antibody 24F.10C12 recognizes CD273 / PD-L2 (also known as B7-DC), a 25 kDa type I transmembrane protein expressed by dendritic cells, activated monocytes and T cells, heart, first trimester placenta, lung and liver, as well as in Hodgkin´s lymphoma cells and primary mediastinal B cell lymphoma (PMBL).

Regulatory Status

RUO

Immunogen

human CD273

Species Reactivity: Human Negative Species:	Applications: Flow Cytometry Immunohistochemistry Functional Application blocking Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	Product Specific References Green MR, Monti S, Rodig SJ, Juszczynski P, Currie T, O'Donnell E, Chapuy B, Takeyama K, Neuberg D, Golub TR, Kutok JL, Shipp MA: Integrative analysis reveals selective 9p24.1 amplification, increased PD-1 ligand expression, and further induction via JAK2 in nodular sclerosing Hodgkin lymphoma and primary mediastinal large B-cell lymphoma. Blood. 2010 Oct 28;116(17):3268-77. [Abstract] [Full Text] Rodríguez-García M, Porichis F, de Jong OG, Levi K, Diefenbach TJ, Lifson JD, Freeman GJ, Walker BD, Kaufmann DE, Kavanagh DG: Expression of PD-L1 and PD-L2 on human macrophages is up-regulated by HIV-1 and differentially modulated by IL-10. J Leukoc Biol. 2011 Apr;89(4):507-15. [Abstract] [Full Text] *Semnani RT, Mahapatra L, Moore V, Sanprasert V, Nutman TB: Functional and phenotypic characteristics of alternative activation induced in human monocytes by interleukin-4 or the parasitic nematode Brugia malayi. Infect Immun. 2011 Oct;79(10):3957-65. [Abstract] [Full Text]

Species Reactivity:

Human

Negative Species:

Applications:

Flow Cytometry
Immunohistochemistry
Functional Application
blocking

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

*Green MR, Monti S, Rodig SJ, Juszczynski P, Currie T, O'Donnell E, Chapuy B, Takeyama K, Neuberg D, Golub TR, Kutok JL, Shipp MA: Integrative analysis reveals selective 9p24.1 amplification, increased PD-1 ligand expression, and further induction via JAK2 in nodular sclerosing Hodgkin lymphoma and primary mediastinal large B-cell lymphoma. Blood. 2010 Oct 28;116(17):3268-77. [Abstract] [Full Text]

*Rodríguez-García M, Porichis F, de Jong OG, Levi K, Diefenbach TJ, Lifson JD, Freeman GJ, Walker BD, Kaufmann DE, Kavanagh DG: Expression of PD-L1 and PD-L2 on human macrophages is up-regulated by HIV-1 and differentially modulated by IL-10. J Leukoc Biol. 2011 Apr;89(4):507-15. [Abstract] [Full Text]

*Semnani RT, Mahapatra L, Moore V, Sanprasert V, Nutman TB: Functional and phenotypic characteristics of alternative activation induced in human monocytes by interleukin-4 or the parasitic nematode Brugia malayi. Infect Immun. 2011 Oct;79(10):3957-65. [Abstract] [Full Text]

For research use only. Not for drug, diagnostic or other use.

Example Data

Fig. 1. Surface staining of CD273 on monocyte-derived dendritic cells with anti-CD273 (24F.10C12) PE.

Fig. 2. Surface staining of CD273 on monocyte-derived dendritic cells with anti-CD273 (24F.10C12) Alexa Fluor^® 647.

Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.

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