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Rabbit Polyclonal to Francisella tularensis ssp. holarctica antigen FTL0187

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Background

The Francisella tularensis subsp. holarctica antigen FLT0187 (cyclohexadienyl dehydratase) is an enzyme involved in biosynthesis of aromatic amino acids. Although poorly characterized, this protein plays an important role in metabolism of the cell.

Specificity

The rabbit polyclonal antibody to FTL0187 reacts with Francisella tularensis subsp. holarctica cyclohexadienyl dehydratase (FTL0187, also known as FTL_0187). The antibody does not react with Francisella tularensis subsp. tularensis homolog.

Regulatory Status

Immunogen

Recombinant FLT0187 (cyclohexadienyl dehydratase) protein produced in E. coli

Species Reactivity:

  • Francisella tularensis ssp. holarctica

Negative Species:

Applications:

  • Western Blotting
  • ELISA
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

References

  • Unpublished.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    FTL0187
    Fig. 1


    Fig. 1. Western blotting analysis of polyclonal anti-Francisella tularensis subsp. holarctica antigen FTL0187.
    St: Mw marker
    FSC200-CL: 10 μg of F. tularensis ssp. holarctica FSC200 cell lysate
    SCHUS4-CL: 10 μg of F. tularensis ssp. tularensis SCHU S4 cell lysate
    FSC200-MF: 10 μg of F. tularensis ssp. holarctica FSC200 membrane fraction
    SCHUS4-CL: 10 μg of F. tularensis ssp. tularensis SCHU S4 membrane fraction
    K1: 50 ng of FTL0187 recombinant protein
    K2: 125 ng of FTL0187 recombinant protein


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