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Mouse Monoclonal to CD49d / Integrin alpha 4

9F10 (IgG1)

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CD49d / integrin alpha 4, unlike other alpha integrins, neither contains an I-domain, nor undergoes disulfide-linked cleavage. It associates with beta 7 chain to form alpha 4 / beta 7 integrin, and with beta 1 chain (CD29) to form VLA-4 integrin. These complexes are important for lymphocyte migration from circulation into tissue (binding VCAM-1) and homing of T cell subsets to Peyer┤s patches (binding MadCAM-1), but VLA-4 is also target for invasive bacteria which contain invasin. CD49d is essential for differentiation and migration of hematopoietic stem cells by their adhesion to bone marrow stromal cells, and provides a costimulatory signal to TCR-CD3 complex by inducing phosphorylation of some focal adhesion proteins.


The mouse monoclonal antibody 9F10 recognizes CD49d (alpha 4 integrin), a 145-180 kDa type I transmembrane glycoprotein expressed on B and T cells, monocytes, eosinophils, basophils, NK cells, and dendritic cells, but not platelets.
HLDA V; WS Code S215

Regulatory Status

Species Reactivity:

  • Human
  • Non-Human Primates
  • Bovine
  • Canine (Dog)
  • Equine (Horse)
  • Feline (Cat)
  • Sheep

Negative Species:


  • Flow Cytometry
  • Immunohistochemistry (frozen sections)
  • Functional Application
    in vitro T cell costimulation
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Stenner MP, Waschbisch A, Buck D, Doerck S, Einsele H, Toyka KV, Wiendl H: Effects of natalizumab treatment on Foxp3+ T regulatory cells. PLoS One. 2008 Oct 6;3(10):e3319. [Abstract] [Full Text]
  • *Chigaev A, Waller A, Amit O, Halip L, Bologa CG, Sklar LA: Real-time analysis of conformation-sensitive antibody binding provides new insights into integrin conformational regulation. J Biol Chem. 2009 May 22;284(21):14337-46 [Abstract] [Full Text]
  • *Schneider-Hohendorf T, Rossaint J, Mohan H, B÷ning D, Breuer J, Kuhlmann T, Gross CC, Flanagan K, Sorokin L, Vestweber D, Zarbock A, Schwab N, Wiendl H: VLA-4 blockade promotes differential routes into human CNS involving PSGL-1 rolling of T cells and MCAM-adhesion of TH17 cells. J Exp Med. 2014 Aug 25;211(9):1833-46. [Abstract] [Full Text]
  • *Yoon SO, Lee IY, Zhang X, Zapata MC, Choi YS: CD9 may contribute to the survival of human germinal center B cells by facilitating the interaction with follicular dendritic cells. FEBS Open Bio. 2014 Apr 13;4:370-6. [Abstract] [Full Text]
  • *Zhang XF, Feng MF: Adherence of human monocytes and NK cells to human TNF-alpha-stimulated porcine endothelial cells. Immunol Cell Biol. 2000 Dec;78(6):633-40. [Abstract] [Full Text]
  • *Soler D, Chapman T, Yang LL, Wyant T, Egan R, Fedyk ER: The binding specificity and selective antagonism of vedolizumab, an anti-alpha4beta7 integrin therapeutic antibody in development for inflammatory bowel diseases. J Pharmacol Exp Ther. 2009 Sep;330(3):864-75. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    9F10 PE FC

    Fig. 1. Surface staining of CD49d in human peripheral blood leukocytes with anti-CD49d (9F10) PE.

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