Rat IgG2a Isotype Control
Technical Information Request Inquiry for Bulk Prices
- In stock! Ready for immediate despatch
- Manufacture on order
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody RTG2A1-1, generated against KLH (keyhole limpet hemocyanin) does not react specifically with any species except from keyhole limpet. Hence all the background that could be observed when working with this antibody on human and other species would be a result of general nonspecific interactions between an rat IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
This rat IgG2a kappa monoclonal antibody (clone RTG2A1-1, also known as G2A-1-1 or KLH/G2A-1-1) detects specifically the hemocyanin of keyhole limpet and can be used as a negative control antibody in other species.
- Flow Cytometry
- Control Experiments
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
Product Specific References
*Berge T, Grønningsæter IH, Lorvik KB, Abrahamsen G, Granum S, Sundvold-Gjerstad V, Corthay A, Bogen B, Spurkland A: SH2D2A modulates T cell mediated protection to a B cell derived tumor in transgenic mice. PLoS One. 2012;7(10):e48239. [Abstract]
*Talkington J, Nickell SP: Role of Fc gamma receptors in triggering host cell activation and cytokine release by Borrelia burgdorferi. Infect Immun. 2001 Jan;69(1):413-9. [Abstract]
*Gillan V, Devaney E: Regulatory T cells modulate Th2 responses induced by Brugia pahangi third-stage larvae. Infect Immun. 2005 Jul;73(7):4034-42. [Abstract] [Full Text]
For research use only. Not for drug, diagnostic or other use.