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Mouse Monoclonal to CD3

SK7 (IgG1)

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  11-173-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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  1P-173-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
  1P-173-T100 PE 100 tests yes choose region PDF datasheetHTML datasheet buy
  PC-173-T025 PerCP 25 tests yes choose region PDF datasheetHTML datasheet buy
  PC-173-T100 PerCP 100 tests yes choose region PDF datasheetHTML datasheet buy
  T9-173-T025 PerCP-Cy™5.5 25 tests yes choose region PDF datasheetHTML datasheet buy
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  T7-173-T025 PE-Cy™7 25 tests yes choose region PDF datasheetHTML datasheet buy
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New T4-173-T025 APC-Cy™7 25 tests yes choose region PDF datasheetHTML datasheet buy
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Background

CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Specificity

The mouse monoclonal antibody SK7 recognizes the CD3 antigen of the TCR/CD3 complex on mature human T cells. This antibody reacts with the epsilon chain of the CD3 complex. The monoclonal antibodies SK7 and UCHT1 recognize overlapping epitopes.
HLDA II; WS Code T118
HLDA III; WS Code T492

Regulatory Status

Immunogen

Human thymocytes

Species Reactivity:

  • Human
  • Non-Human Primates

Negative Species:

Applications:

  • Flow Cytometry
  • Western Blotting
  • Immunohistochemistry (frozen sections)
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Wood GS, Burns BF, Dorfman RF, Warnke RA: The immunohistology of non-T cells in the acquired immunodeficiency syndrome. Am J Pathol. 1985 Sep;120(3):371-9. [Abstract]
  • *Li B, Wang H, Dai J, Ji J, Qian W, Zhang D, Hou S, Guo Y: Construction and characterization of a humanized anti-human CD3 monoclonal antibody 12F6 with effective immunoregulation functions. Immunology. 2005 Dec;116(4):487-98. [Abstract] [Full Text]
  • *Recio MJ, Moreno-Pelayo MA, Kiliç SS, Guardo AC, Sanal O, Allende LM, Pérez-Flores V, Mencía A, Modamio-Høybjør S, Seoane E, Regueiro JR: Differential biological role of CD3 chains revealed by human immunodeficiencies. J Immunol. 2007 Feb 15;178(4):2556-64. [Abstract] [Full Text]
  • *Guttman-Yassky E, Vugmeyster Y, Lowes MA, Chamian F, Kikuchi T, Kagen M, Gilleaudeau P, Lee E, Hunte B, Howell K, Dummer W, Bodary SC, Krueger JG: Blockade of CD11a by efalizumab in psoriasis patients induces a unique state of T-cell hyporesponsiveness. J Invest Dermatol. 2008 May;128(5):1182-91. [Abstract] [Full Text]
  • *Qiao D, Li L, Guo J, Lao S, Zhang X, Zhang J, Wu C: Mycobacterium tuberculosis culture filtrate protein 10-specific effector/memory CD4⁺ and CD8⁺ T cells in tubercular pleural fluid, with biased usage of T cell receptor Vβ chains. Infect Immun. 2011 Aug;79(8):3358-65. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    SK7 PerCP-Cy5-5


    Fig. 1. Surface staining of human peripheral blood with anti-human CD3 (SK7) PerCP-CyTM5.5.


    SK7 APC-Cy7


    Fig. 2. Surface staining of human peripheral blood with anti-human CD3 (SK7) APC-CyTM7.


    SK7 PE-Cy7


    Fig. 3. Surface staining of human peripheral blood with anti-human CD3 (SK7) PE-CyTM7.

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