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Mouse Monoclonal to CD22

S-HCL-1 (IgG2b)

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  11-175-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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  1P-175-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
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  1A-175-T025 APC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1A-175-T100 APC 100 tests yes choose region PDF datasheetHTML datasheet buy
  T9-175-T025 PerCP-Cy™5.5 25 tests yes choose region PDF datasheetHTML datasheet buy
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  T7-175-T025 PE-Cy™7 25 tests yes choose region PDF datasheetHTML datasheet buy
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Background

CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.

Specificity

The mouse monoclonal antibody S-HCL-1 (also known as S-HCL1) recognizes CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes.
HLDA IV; WS Code B48

Regulatory Status

Immunogen

Whole hairy cell leukemia cells and membrane preparation

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Foon KA, Todd RF 3rd: Immunologic classification of leukemia and lymphoma. Blood. 1986 Jul;68(1):1-31. [Abstract]
  • *Reineks EZ, Osei ES, Rosenberg A, Auletta J, Meyerson HJ: CD22 expression on blastic plasmacytoid dendritic cell neoplasms and reactivity of anti-CD22 antibodies to peripheral blood dendritic cells. Cytometry B Clin Cytom. 2009 Jul;76(4):237-48 [Abstract]
  • *Huang J, Fan G, Zhong Y, Gatter K, Braziel R, Gross G, Bakke A: Diagnostic usefulness of aberrant CD22 expression in differentiating neoplastic cells of B-Cell chronic lymphoproliferative disorders from admixed benign B cells in four-color multiparameter flow cytometry. Am J Clin Pathol. 2005 Jun;123(6):826-32. [Abstract]
  • *James SE, Greenberg PD, Jensen MC, Lin Y, Wang J, Till BG, Raubitschek AA, Forman SJ, Press OW: Antigen sensitivity of CD22-specific chimeric TCR is modulated by target epitope distance from the cell membrane. J Immunol. 2008 May 15;180(10):7028-38. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    S-HCL-1 APC FCIC


    Fig. 1. Intracellular staining of CD22 in human immature B cells with anti-CD22 (S-HCL-1) APC.



    S-HCL-1 PC55


    Fig. 2. Surface staining of CD22 in human peripheral blood with anti-CD22 (S-HCL-1) PerCP-CyTM5.5.


    S-HCL-1 PE-Cy7


    Fig. 3. Surface staining of CD22 in human peripheral blood with anti-CD22 (S-HCL-1) PE-CyTM7.

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