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Mouse Monoclonal to Ikaros

4E9 (IgG1)

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  11-159-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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Background

Ikaros, also known as IKZF1 (Ikaros family zinc finger protein 1) is a hematopoietic-specific transcription factor involved in the regulation of lymphocyte development, together with other members of this family, such as Aiolos and Helios. Ikaros forms homo- and heterodimers with these proteins and functions predominantly in early hematopoietic development. Expression of Ikaros, Aiolos and Helios is restricted to cells of the hematopoietic system, whereas other family members, Eos and Pegassus, are more widely expressed. Disruption of Ikaros leads to T and B cell leukemias.

Specificity

The mouse monoclonal antibody 4E9 recognizes Ikaros, a transcription factor expressed broadly in hematopoietic progenitors and serving as a key regulator of lymphopoiesis.

Regulatory Status

Immunogen

Recombinant human Ikaros (C-terminal part)

Species Reactivity:

  • Human
  • Mouse

Negative Species:

Applications:

  • Flow Cytometry
    Application note: intracellular staining
  • Immunoprecipitation
  • Western Blotting
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Koipally J, Heller EJ, Seavitt JR, Georgopoulos K: Unconventional potentiation of gene expression by Ikaros. J Biol Chem. 2002 Apr 12;277(15):13007-15. [Abstract] [Full Text]
  • *López-Nieva P, Santos J, Fernández-Piqueras J: Defective expression of Notch1 and Notch2 in connection to alterations of c-Myc and Ikaros in gamma-radiation-induced mouse thymic lymphomas. Carcinogenesis. 2004 Jul;25(7):1299-304. [Abstract] [Full Text]
  • *Loeper S, Asa SL, Ezzat S: Ikaros modulates cholesterol uptake: a link between tumor suppression and differentiation. Cancer Res. 2008 May 15;68(10):3715-23. [Abstract] [Full Text]
  • *Elliott J, Jolicoeur C, Ramamurthy V, Cayouette M: Ikaros confers early temporal competence to mouse retinal progenitor cells. Neuron. 2008 Oct 9;60(1):26-39. [Abstract]
  • *Kathrein KL, Lorenz R, Innes AM, Griffiths E, Winandy S: Ikaros induces quiescence and T-cell differentiation in a leukemia cell line. Mol Cell Biol. 2005 Mar;25(5):1645-54. [Abstract] [Full Text]
  • *Schwickert TA, Tagoh H, Gültekin S, Dakic A, Axelsson E, Minnich M, Ebert A, Werner B, Roth M, Cimmino L, Dickins RA, Zuber J, Jaritz M, Busslinger M: Stage-specific control of early B cell development by the transcription factor Ikaros. Nat Immunol. 2014 Mar;15(3):283-93. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    4E9 PE FC


    Fig. 1. Intracellular staining of Ikaros in human peripheral blood leukocytes with anti-Ikaros (4E9) PE.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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