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Mouse Monoclonal to CD49b / Integrin alpha 2

AK7 (IgG1)

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Product No. Form Quantity Stock Price Datasheet Shop
  11-151-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-151-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-151-T025 FITC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1F-151-T100 FITC 100 tests yes choose region PDF datasheetHTML datasheet buy
  1P-151-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
  1P-151-T100 PE 100 tests yes choose region PDF datasheetHTML datasheet buy
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Background

CD49b / Integrin alpha 2 (ITGA2; alpha 2 subunit of VLA-2) heterodimerizes with CD29 to form the VLA-2 integrin alpha2/beta1 complex, which serves as a receptor for type I collagen, laminin, and E-cadherin. It also acts as a receptor for echovirus. Expressed on platelets, activated lymphocytes, monocytes, fibroblasts, epitelial and endothelial cells, CD49b mediates adhesion of these cells to the extracellular matrix. This interaction supports e.g. T cell proliferation and cytokine production, or wound healing. Defect in CD49b is associated with bleeding disorder platelet-type 9. Antibodies against CD49b are found in several immune disorders, including neonatal alloimmune thrombocytopenia.

Specificity

The mouse monoclonal antibody AK7 recognizes CD49b, a 160-165 kDa alpha subunit of VLA-2 integrin complex expressed on platelets, megakaryocytes, activated T and B cells, monocytes, epithelial cells, endothelial cells and fibroblasts.
HLDA Workshop code V S190

Regulatory Status

Immunogen

Human platelets

Species Reactivity:

  • Human
  • Non-Human Primates

Negative Species:

Applications:

  • Flow Cytometry
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry (frozen sections)
  • Immunocytochemistry
  • ELISA
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Gamble JR, Matthias LJ, Meyer G, Kaur P, Russ G, Faull R, Berndt MC, Vadas MA: Regulation of in vitro capillary tube formation by anti-integrin antibodies. J Cell Biol. 1993 May;121(4):931-43. [Abstract] [Full Text]
  • *Londrigan SL, Hewish MJ, Thomson MJ, Sanders GM, Mustafa H, Coulson BS: Growth of rotaviruses in continuous human and monkey cell lines that vary in their expression of integrins. J Gen Virol. 2000 Sep;81(Pt 9):2203-13. [Abstract] [Full Text]
  • *Werr J, Johansson J, Eriksson EE, Hedqvist P, Ruoslahti E, Lindbom L: Integrin alpha(2)beta(1) (VLA-2) is a principal receptor used by neutrophils for locomotion in extravascular tissue. Blood. 2000 Mar 1;95(5):1804-9. [Abstract] [Full Text]
  • *Ammon C, Meyer SP, Schwarzfischer L, Krause SW, Andreesen R, Kreutz M: Comparative analysis of integrin expression on monocyte-derived macrophages and monocyte-derived dendritic cells. Immunology. 2000 Jul;100(3):364-9. [Abstract] [Full Text]
  • *Ciarlet M, Crawford SE, Cheng E, Blutt SE, Rice DA, Bergelson JM, Estes MK: VLA-2 (alpha2beta1) integrin promotes rotavirus entry into cells but is not necessary for rotavirus attachment. J Virol. 2002 Feb;76(3):1109-23. [Abstract]
  • *Kögler G, Sensken S, Airey JA, Trapp T, Müschen M, Feldhahn N, Liedtke S, Sorg RV, Fischer J, Rosenbaum C, Greschat S, Knipper A, Bender J, Degistirici O, Gao J, Caplan AI, Colletti EJ, Almeida-Porada G, Müller HW, Zanjani E, Wernet P: A new human somatic stem cell from placental cord blood with intrinsic pluripotent differentiation potential. J Exp Med. 2004 Jul 19;200(2):123-35. [Abstract] [Full Text]
  • *Müller K, Schinn M, Reichrath J, Meineke V: 1alpha,25-Dihydroxyvitamin D3 modulates the response of human keratinocytes to ionizing radiation exposure. Anticancer Res. 2006 Jul-Aug;26(4A):2735-41. [Abstract] [Full Text]
  • *Engl T, Makareviæ J, Relja B, Natsheh I, Müller I, Beecken WD, Jonas D, Blaheta RA: Mycophenolate mofetil modulates adhesion receptors of the beta1 integrin family on tumor cells: impact on tumor recurrence and malignancy. BMC Cancer. 2005 Jan 11;5:4. [Abstract] [Full Text]
  • *Sutavani RV, Bradley RG, Ramage JM, Jackson AM, Durrant LG, Spendlove I: CD55 costimulation induces differentiation of a discrete T regulatory type 1 cell population with a stable phenotype. J Immunol. 2013 Dec 15;191(12):5895-903. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    AK7 purified FC


    Fig. 1. Surface staining of CD49b in human peripheral blood with anti-CD49b (AK7) purified / GAM-APC.


    AK7 PE FC


    Fig. 2. Surface staining of CD49b in human peripheral blood with anti-CD49b (AK7) PE.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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