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Mouse Monoclonal to Ki-67

Ki-67 (IgG1)

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Background

Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies.

Specificity

The mouse monoclonal antibody Ki-67 recognizes Ki-67 antigen, a non-histone nuclear protein expressed exclusively in proliferating cells.

Regulatory Status

Immunogen

Nuclei of the Hodgkin lymphoma cell line L428

Species Reactivity:

  • Human
  • Bovine

Negative Species:

Applications:

  • Flow Cytometry
    Intracellular staining
  • Western Blotting
  • Immunohistochemistry
  • Immunocytochemistry
    Application note: paraformaldehyde fixation; recommended antibody concentration 1 μg/ml
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Gerdes J, Schwab U, Lemke H, Stein H: Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 1983 Jan 15;31(1):13-20. [Abstract]
  • *Gerdes J: Ki-67 and other proliferation markers useful for immunohistological diagnostic and prognostic evaluations in human malignancies. Semin Cancer Biol. 1990 Jun;1(3):199-206. [Abstract]
  • *Gerdes J, Lemke H, Baisch H, Wacker HH, Schwab U, Stein H: Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. J Immunol. 1984 Oct;133(4):1710-5. [Abstract]
  • *Duchrow M, Schlüter C, Key G, Kubbutat MH, Wohlenberg C, Flad HD, Gerdes J: Cell proliferation-associated nuclear antigen defined by antibody Ki-67: a new kind of cell cycle-maintaining proteins. Arch Immunol Ther Exp (Warsz). 1995;43(2):117-21. [Abstract]
  • *Schlüter C, Duchrow M, Wohlenberg C, Becker MH, Key G, Flad HD, Gerdes J: The cell proliferation-associated antigen of antibody Ki-67: a very large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell cycle-maintaining proteins. J Cell Biol. 1993 Nov;123(3):513-22. [Abstract]
  • *And many other.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    Ki-67 ICC


    Fig. 1. Immunocytochemistry detection of Ki-67 in U2OS cell line (human osteosarcoma) using monoclonal antibody Ki-67 (green). Cell nuclei stained with DAPI (blue).

    Ki-67 FC


    Fig. 2. Flow cytometry analysis of human peripheral blood mononuclear cells stimulated with PHA. Surface staining of CD25 (clone MEM-181 APC) was followed by permeabilization and nuclear staining of Ki-67 (clone Ki-67 PE).

    Ki-67 AF6


    Fig. 3. Surface staining of PHA-stimulated (for 3 days) PBMC with anti-Ki-67 (Ki-67) Alexa Fluor® 647.


    Ki-67 AF488


    Fig. 4. Surface staining of Jurkat cells with anti-Ki-67 (Ki-67) Alexa Fluor® 488.


    Ki-67 PC7


    Fig. 5. Surface staining of HEK-293 cells with anti-Ki-67 (Ki-67) PE-CyTM7.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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