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Mouse Monoclonal to CD65

VIM8 (IgM)

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CD65 is a fucosylated carbohydrate antigen (ceramide-dodecasaccharide, type II fucoganglioside), which serves as a ligand for CD62E (E-selectin). Its structure is Gal beta1-4 GlcNAc beta1-3 Gal beta1-4 GlcNAc (3-1 Fuc alpha) beta1-3 ceramide. Unlike CD65s, the CD65 antigen does not contain terminal sialic acid, the rest of their structure is identical. CD65 is expressed on granulocytes and monocytes and participates in cell adhesion. It has been reported as important for extravascular infiltration of acute monocytic leukemia cells.


The mouse monoclonal antibody VIM8 recognizes human CD65, an asialo-fucoganglioside expressed on peripheral blood granulocytes (highly) and monocytes (weakly).
HLDA II; WS Code M 27
HLDA III; WS Code M 261
HLDA IV; WS Code M 23
HLDA V; WS Code MA095, MA096

Regulatory Status


THP-1 cell line

Species Reactivity:

  • Human

Negative Species:


  • Flow Cytometry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Paietta E, Neuberg D, Bennett JM, Dewald G, Rowe JM, Cassileth PA, Cripe L, Tallman MS, Wiernik PH and the Eastern Cooperative Oncology Group: Low expression of the myeloid differentiation antigen CD65s, a feature of poorly differentiated AML in older adults: study of 711 patients enrolled in ECOG trials. Leukemia. 2003 Aug;17(8):1544-50. [Abstract]
  • *Kniep B, Peter-Katalinic J, Müthing J, Majdic O, Pickl WF, Knapp W: The CDw65 monoclonal antibodies VIM-8 and VIM-11 bind to the neutral glycolipid V3FucnLc8Cer. J Biochem. 1996 Mar;119(3):456-62. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data


    Fig. 1. Surface staining of CD65 in human peripheral blood with anti-CD65 (VIM8) FITC.

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