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Mouse Monoclonal to CD172a/b

SE5A5 (IgG1)

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Background

CD172a, the signal-regulatory protein alpha (SIRP alpha), also known as SH2 domain-containing phosphatase substrate-1 (SHPS1), is a 75-110 kDa transmembrane glycoprotein expressed mainly on granulocytes, monocytes, macrophages, dendritic cells and neurons. Its extracellular ligand is CD47. CD172a serves as a substrate of activated receptor tyrosine kinases and upon phosphorylation it recruits SH2 domain-containing tyrosine phosphatases, thereby regulating signal transduction processes related to cell activation, transmigration and phagocytosis. CD172a is a specific marker of cardiomyocytes derived from human pluripotent stem cells and serves as a negative regulator of signaling and growth in myeloid progenitor cells.

Specificity

The mouse monoclonal antibody SE5A5 recognizes a common epitope on human CD172a and CD172b antigens (approx. 90 kDa and approx. 50 kDa, respectively), although its reactivity with CD172a is higher.
HLDA VII; WS Code 70259

Regulatory Status

Immunogen

NIH-3T3 / human CD172a cell line

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Florian S, Ghannadan M, Mayerhofer M, Aichberger KJ, Hauswirth AW, Schernthaner GH, Printz D, Fritsch G, Böhm A, Sonneck K, Krauth MT, Müller MR, Sillaber C, Sperr WR, Bühring HJ, Valent P: Evaluation of normal and neoplastic human mast cells for expression of CD172a (SIRPalpha), CD47, and SHP-1. J Leukoc Biol. 2005 Jun;77(6):984-92. Epub 2005 Mar 22. [Abstract] [Full Text]
  • *Ha B, Lv Z, Bian Z, Zhang X, Mishra A, Liu Y: 'Clustering' SIRPα into the plasma membrane lipid microdomains is required for activated monocytes and macrophages to mediate effective cell surface interactions with CD47. PLoS One. 2013 Oct 15;8(10):e77615. [Abstract] [Full Text]
  • *Hatherley D, Harlos K, Dunlop DC, Stuart DI, Barclay AN: The structure of the macrophage signal regulatory protein alpha (SIRPalpha) inhibitory receptor reveals a binding face reminiscent of that used by T cell receptors. J Biol Chem. 2007 May 11;282(19):14567-75. [Abstract] [Full Text]
  • *Brooke G, Holbrook JD, Brown MH, Barclay AN: Human lymphocytes interact directly with CD47 through a novel member of the signal regulatory protein (SIRP) family. J Immunol. 2004 Aug 15;173(4):2562-70. [Abstract] [Full Text]
  • *Kim EJ, Suk K, Lee WH: SHPS-1 and a synthetic peptide representing its ITIM inhibit the MyD88, but not TRIF, pathway of TLR signaling through activation of SHP and PI3K in THP-1 cells. Inflamm Res. 2013 Apr;62(4):377-86. [Abstract]
  • *Schernthaner GH, Hauswirth AW, Baghestanian M, Agis H, Ghannadan M, Worda C, Krauth MT, Printz D, Fritsch G, Sperr WR, Valent P: Detection of differentiation- and activation-linked cell surface antigens on cultured mast cell progenitors. Allergy. 2005 Oct;60(10):1248-55. [Abstract]
  • *Orazizadeh M, Salter DM: The expression of signal regulatory protein-alpha in normal and osteoarthritic human articular cartilage and its involvement in chondrocyte mechano-transduction response. Iran Biomed J. 2007 Apr;11(2):119-24. [Abstract]
  • *Suparak S, Muangsombut V, Riyapa D, Stevens JM, Stevens MP, Lertmemongkolchai G, Korbsrisate S: Burkholderia pseudomallei-induced cell fusion in U937 macrophages can be inhibited by monoclonal antibodies against host cell surface molecules. Microbes Infect. 2011 Nov;13(12-13):1006-11. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    SE5A5 pur


    Fig. 1. Surface staining of human peripheral blood cells with anti-human CD172a/b (SE5A5) purified, GAM-APC.


    SE5A5 PE


    Fig. 2. Surface staining of human peripheral blood cells with anti-human CD172a/b (SE5A5) PE.

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