System Certification ISO 9001:2000
Exbio Antibodies CZ / SK eShop
by categories | a-z list | Application type: Flow Cytometry | Immunohistochemistry | Functional Studies

Mouse Monoclonal to CD164

67D2 (IgG1)

Technical Information Request    Inquiry for Bulk Prices

Product No. Form Quantity Stock Price Datasheet Shop
New 11-135-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
New 1P-135-T100 PE 100 tests yes choose region PDF datasheetHTML datasheet buy
- In stock! Ready for immediate despatch - Manufacture on order

Background

CD164, also known as endolyn, is a type I transmembrane protein with heavily glycosylated extracellular part containing sialic acid and glycosaminoglycan residues. CD164 plays both adhesive and antiadhesive role and serves as a potent negative regulator for CD34+ CD38- hematopoietic progenitor cell proliferation. It has also been reported to be involved in myogenic differentiation and cancer metastasis. The adhesive and negative regulatory functions seem to depend on different posttranslational modifications of CD164 protein.

Specificity

The mouse monoclonal antibody 67D2 recognizes class III epitope (not sensitive to sialidase, N-glycanase, O-glycosidase, and O-sialoglycoprotease) of CD164, a sialomucin expressed in hematopoietic myeloid and erythroid progenitors, activated basophils, and in various carcinomas and leukemic cells.
HLDA 8

Regulatory Status

Immunogen

Breast tumor cell line T-47D

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
  • Immunoprecipitation
  • Western Blotting
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Jorgensen-Tye B, Levesque JP, Royle L, Doyonnas R, Chan JY, Dwek RA, Rudd PM, Harvey DJ, Simmons PJ, Watt SM: Epitope recognition of antibodies that define the sialomucin, endolyn (CD164), a negative regulator of haematopoiesis. Tissue Antigens. 2005 Mar;65(3):220-39. [Abstract]
  • *Pilling D, Vakil V, Gomer RH: Improved serum-free culture conditions for the differentiation of human and murine fibrocytes. J Immunol Methods. 2009 Dec 31;351(1-2):62-70. [Abstract] [Full Text]
  • *Hennersdorf F, Florian S, Jakob A, Baumgärtner K, Sonneck K, Nordheim A, Biedermann T, Valent P, Bühring HJ: Identification of CD13, CD107a, and CD164 as novel basophil-activation markers and dissection of two response patterns in time kinetics of IgE-dependent upregulation. Cell Res. 2005 May;15(5):325-35. [Abstract]
  • *Doyonnas R, Yi-Hsin Chan J, Butler LH, Rappold I, Lee-Prudhoe JE, Zannettino AC, Simmons PJ, Bühring HJ, Levesque JP, Watt SM: CD164 monoclonal antibodies that block hemopoietic progenitor cell adhesion and proliferation interact with the first mucin domain of the CD164 receptor. J Immunol. 2000 Jul 15;165(2):840-51. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    67D2 pur


    Fig. 1. Surface staining of human peripheral blood cells using anti-CD164 (67D2) purified, GAM-APC.


    67D2 PE


    Fig. 2. Surface staining of human peripheral blood cells using anti-CD164 (67D2) PE.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
    © 2003 EXBIO. All rights reserved.
    terms and conditions | privacy policy