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Mouse Monoclonal to CD100

133-1C6 (IgM)

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Background

CD100, also known as Semaphorin 4D, is a homodimerizing type I transmembrane glycoprotein containing an extracellular semaphorin domain. It is expressed on most hematopoietic cells with the exception of immature bone marrow cells, erythrocytes and platelets. A 120 kDa soluble form is generated from the transmembrane form by proteolytic cascade following primary T and B cell activation. It seems CD100 acts through dampening CD72-mediated negative signaling. CD100 promotes angiogenesis, invasive growth, proliferation and anti-apoptosis of cancer cells in vitro. Higher expression levels of CD100 correlate with poor survival in soft tissue sarcoma patients.

Specificity

The mouse monoclonal antibody 133-1C6 recognizes CD100, an approximately 150 kDa (when reduced) semaphorin family member expressed mainly on lymphocytes, NK cells, monocytes/macrophages and granulocytes, but also on some non-hematopoietic cells.
HLDA VI.; WS Code N-L026

Regulatory Status

Immunogen

PHA stimulated human PBL

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
  • Immunoprecipitation
  • ELISA
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
  • Jiang X, Björkström NK, Melum E: Intact CD100-CD72 Interaction Necessary for TCR-Induced T Cell Proliferation. Intact CD100-CD72 Interaction Necessary for TCR-Induced T Cell Proliferation. Front Immunol. 2017 Jun 30;8:765. doi: 10.3389/fimmu.2017.00765. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    133-1C6 pur

    Fig. 1. Surface staining of human peripheral blood with anti-CD100 (133-1C6) purified, GAM-APC.


    133-1C6 PE

    Fig. 2. Surface staining of human peripheral blood with anti-CD100 (133-1C6) PE.

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