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Mouse Monoclonal to CD154 / CD40L

24-31 (IgG1)

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  11-781-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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Background

CD154 / CD40L (CD40 ligand) is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. Its counter-receptor CD40 is expressed on antigen presenting cells, including dendritic cells, macrophages, and B cells, and also on fibroblasts. Triggering of CD40 by CD40L causes maturation of dendritic cells and upregulation of antigen presentation in functions of the MHC and costimulatory molecules. CD40L also functions as a direct stimulating factor for T cells. CD40L plays also roles e.g. in antibody class switching and modulation of apoptosis in the germinal center.

Specificity

The mouse monoclonal antibody 24-31 detects CD154 / CD40L (CD40-ligand), a 39 kDa cell surface type II glycoprotein expressed predominantly on activated CD4+ lymphocytes.

Regulatory Status

Immunogen

human CD154 fusion protein

Species Reactivity:

  • Human
  • Non-Human Primates

Negative Species:

Applications:

  • Flow Cytometry
  • Immunocytochemistry
  • Functional Application
    blocking
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Kum WW, Hung RW, Cameron SB, Chow AW: Temporal sequence and functional implications of V beta-specific T cell receptor down-regulation and costimulatory molecule expression following in vitro stimulation with the staphylococcal superantigen Toxic shock syndrome toxin-1. J Infect Dis. 2002 Feb 15;185(4):555-60 [Abstract] [Full Text]
  • *Fernandes MS, Gomes EM, Butcher LD, Hernandez-Alcoceba R, Chang D, Kansopon J, Newman J, Stone MJ, Tong AW: Growth inhibition of human multiple myeloma cells by an oncolytic adenovirus carrying the CD40 ligand transgene. Clin Cancer Res. 2009 Aug 1;15(15):4847-56. [Abstract] [Full Text]
  • *Barnhart B, Ford GS, Bhushan A, Song C, Covey LR: A polymorphic CD40 ligand (CD154) molecule mediates CD40-dependent signalling but interferes with the ability of soluble CD40 to functionally block CD154:CD40 interactions. Immunology. 2000 Jan;99(1):54-61. [Abstract] [Full Text]
  • *Grammer AC, McFarland RD, Heaney J, Darnell BF, Lipsky PE: Expression, regulation, and function of B cell-expressed CD154 in germinal centers. J Immunol. 1999 Oct 15;163(8):4150-9. [Abstract] [Full Text]
  • *Kornbluth RS, Kee K, Richman DD: CD40 ligand (CD154) stimulation of macrophages to produce HIV-1-suppressive beta-chemokines. Proc Natl Acad Sci U S A. 1998 Apr 28;95(9):5205-10. [Abstract] [Full Text]
  • *Berner B, Wolf G, Hummel KM, Müller GA, Reuss-Borst MA: Increased expression of CD40 ligand (CD154) on CD4+ T cells as a marker of disease activity in rheumatoid arthritis. Ann Rheum Dis. 2000 Mar;59(3):190-5. [Abstract] [Full Text]
  • *Brams P, Black A, Padlan EA, Hariharan K, Leonard J, Chambers-Slater K, Noelle RJ, Newman R: A humanized anti-human CD154 monoclonal antibody blocks CD154-CD40 mediated human B cell activation. Int Immunopharmacol. 2001 Feb;1(2):277-94. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    24-31 FITC


    Fig. 1. Flow cytometry detection of CD154 / CD40L in IgE-activated human peripheral blood with anti-CD154 / CD40L (24-31) FITC. Orange = blank, blue = CD154 negative cells, red = CD154 positive cells.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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