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categories: Apoptosis (Human)

Mouse Monoclonal to DDIT4L

DDIT-03 (IgG1)

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  11-725-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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Background

DDIT4L (DNA-damage-inducible transcript 4-like), also known as REDD2 (regulated in development and DNA damage response 2) or RTP801L is a stress-inducted protein, which was shown to mediate monocyte cell death through a reduction in thioredoxin-1 expression, and is highly expressed in atherosclerotic lesions. Stimulation of DDIT4L expression in macrophages increases oxidized LDL-induced macrophage death.

Specificity

The mouse monoclonal antibody DDIT-03 recognizes DDIT4L / REDD2 protein, which belongs to stress-induced proteins involved in mediation of cell death.

Regulatory Status

Immunogen

N-terminal recombinant fragment of human DDIT4L (amino acids 2-98)

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
    Application note: intracellular staining
  • Western Blotting
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Imen JS, Billiet L, Cuaz-Pérolin C, Michaud N, Rouis M: The regulated in development and DNA damage response 2 (REDD2) gene mediates human monocyte cell death through a reduction in thioredoxin-1 expression. Free Radic Biol Med. 2009 May 15;46(10):1404-10 [Abstract]
  • *Corradetti MN, Inoki K, Guan KL: The stress-inducted proteins RTP801 and RTP801L are negative regulators of the mammalian target of rapamycin pathway. J Biol Chem. 2005 Mar 18;280(11):9769-72. [Abstract] [Full Text]
  • *Cuaz-Pérolin C, Furman C, Larigauderie G, Legedz L, Lasselin C, Copin C, Jaye M, Searfoss G, Yu KT, Duverger N, Nègre-Salvayre A, Fruchart JC, Rouis M.REDD2 gene is upregulated by modified LDL or hypoxia and mediates human macrophage cell death. Arterioscler Thromb Vasc Biol. 2004 Oct;24(10):1830-5. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    DDIT
    Fig. 1.


    Fig. 1. Western blotting analysis of DDIT4L expression in HEK293-DDIT4L transfectants and HEK293 cells using mouse monoclonal antibody DDIT-03.


    1P-725


    Fig. 1. Flow cytometry detection of DDIT4L in DDIT4L-transfected Hek293 cells using the mouse monoclonal antibody DDIT-03 PE.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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