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Mouse Monoclonal to CD235a / Glycophorin A

JC159 (IgG1)

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Product No. Form Quantity Stock Price Datasheet Shop
  11-784-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-784-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-784-T025 FITC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1F-784-T100 FITC 100 tests yes choose region PDF datasheetHTML datasheet buy
  1P-784-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
  1P-784-T100 PE 100 tests yes choose region PDF datasheetHTML datasheet buy
  1A-784-T025 APC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1A-784-T100 APC 100 tests yes choose region PDF datasheetHTML datasheet buy
  T8-784-T025 PE-Cy™5 25 tests yes choose region PDF datasheetHTML datasheet buy
  T8-784-T100 PE-Cy™5 100 tests yes choose region PDF datasheetHTML datasheet buy
  T4-784-T025 APC-Cy™7 25 tests yes choose region PDF datasheetHTML datasheet buy
  T4-784-T100 APC-Cy™7 100 tests yes choose region PDF datasheetHTML datasheet buy
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Background

CD235a (Glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to glycophorin B (GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, an Streptococcus adhesin.

Specificity

The mouse monoclonal antibody JC159 recognizes an epitope between amino acids 27 and 40 of the extracellular portion of CD235a (glycophorin A), a sialoglycoprotein expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL. The antibody does not react with glycophorin B.

Regulatory Status

Immunogen

Membrane preparation from splenic hairy cell leukemia

Species Reactivity:

  • Human
  • Rat

Negative Species:

Applications:

  • Flow Cytometry
  • Immunohistochemistry (paraffin sections)
    Recommended dilution:10 μg/ml
  • Immunohistochemistry (frozen sections)
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Maijenburg MW, Kleijer M, Vermeul K, Mul EP, van Alphen FP, van der Schoot CE, Voermans C: The composition of the mesenchymal stromal cell compartment in human bone marrow changes during development and aging. Haematologica. 2012 Feb;97(2):179-83. [Abstract] [Full Text]
  • *Beck Z, Jagodzinski LL, Eller MA, Thelian D, Matyas GR, Kunz AN, Alving CR: Platelets and erythrocyte-bound platelets bind infectious HIV-1 in plasma of chronically infected patients. PLoS One. 2013 Nov 25;8(11):e81002. [Abstract] [Full Text]
  • *Yamauchi T, Takenaka K, Urata S, Shima T, Kikushige Y, Tokuyama T, Iwamoto C, Nishihara M, Iwasaki H, Miyamoto T, Honma N, Nakao M, Matozaki T, Akashi K: Polymorphic Sirpa is the genetic determinant for NOD-based mouse lines to achieve efficient human cell engraftment. Blood. 2013 Feb 21;121(8):1316-25. [Abstract] [Full Text]
  • *Alijotas-Reig J, Palacio-Garcia C, Llurba E, Vilardell-Tarres M: Cell-derived microparticles and vascular pregnancy complications: a systematic and comprehensive review. Fertil Steril. 2013 Feb;99(2):441-9. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    JC159 PE


    Fig. 1. Surface staining of CD235a in human peripheral blood (erythrocytes and leukocytes) with anti-CD235a (JC159) PE.


    JC159 PE-Cy5


    Fig. 2. Surface staining of CD235a in human peripheral blood (erythrocytes and leukocytes) with anti-CD235a (JC159) PE-CyTM5.


    JC159 FITC


    Fig. 3. Surface staining of CD235a in human peripheral blood (erythrocytes and leukocytes) with anti-CD235a (JC159) FITC.


    JC159 APC-Cy7


    Fig. 4. Surface staining of CD235a in human peripheral blood (erythrocytes and leukocytes) with anti-CD235a (JC159) APC-CyTM7.


    JC159 purified


    Fig. 5. Surface staining of CD235a in human peripheral blood (erythrocytes and leukocytes) with anti-CD235a (JC159) purified, GAM-APC.


    1A-784


    Fig. 6. Surface staining of CD235a in human peripheral blood (erythrocytes and leukocytes) with anti-CD235a (JC159) APC.

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