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Mouse Monoclonal to CD178 / Fas-L

NOK-1 (IgG1)

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  11-754-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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Background

CD178 / Fas-L (Fas ligand, CD95L), a member of TNF family transmembrane glycoproteins, is responsible for induction of apoptosis in cells containing its receptor CD95 / Fas. The CD178-mediated apoptosis pathway has been implicated in peripheral tolerance, tissue pathology, and maintenance of the immune privileged sites. Defects in this interaction may be related to some cases of systemic lupus erythematosus (SLE). CD178 was also described as a co-stimulatory receptor for T-cell activation in mice in vivo.

Specificity

The mouse monoclonal antibody NOK-1 recognizes CD178 / Fas-L, an approximately 40 kDa transmembrane glycoprotein expressed on neutrophils, monocytes, and activated T and NK cells.

HCDM Workshop: VII 70322

Regulatory Status

Immunogen

L5178Y mouse T lymphoma cells expressing recombinant human CD178

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
  • Immunoprecipitation
  • Western Blotting
  • Immunocytochemistry
  • Functional Application
    blocking
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Kayagaki N, Kawasaki A, Ebata T, Ohmoto H, Ikeda S, Inoue S, Yoshino K, Okumura K, Yagita H: Metalloproteinase-mediated release of human Fas ligand. J Exp Med. 1995 Dec 1;182(6):1777-83. [Abstract] [Full Text]
  • *Jodo S, Pidiyar VJ, Xiao S, Furusaki A, Sharma R, Koike T, Ju ST: Fas ligand (CD178) cytoplasmic tail is a positive regulator of Fas ligand-mediated cytotoxicity. J Immunol. 2005 Apr 15;174(8):4470-4. [Abstract] [Full Text]
  • *Ghadimi MP, Sanzenbacher R, Thiede B, Wenzel J, Jing Q, Plomann M, Borkhardt A, Kabelitz D, Janssen O: Identification of interaction partners of the cytosolic polyproline region of CD95 ligand (CD178). FEBS Lett. 2002 May 22;519(1-3):50-8. [Abstract]
  • *Chopin D, Barei-Moniri R, Maillé P, Le Frère-Belda MA, Muscatelli-Groux B, Merendino N, Lecerf L, Stoppacciaro A, Velotti F: Human urinary bladder transitional cell carcinomas acquire the functional Fas ligand during tumor progression. Am J Pathol. 2003 Apr;162(4):1139-49. [Abstract] [Full Text]
  • *Ji J, Chen JJ, Braciale VL, Cloyd MW: Apoptosis induced in HIV-1-exposed, resting CD4+ T cells subsequent to signaling through homing receptors is Fas/Fas ligand-mediated. J Leukoc Biol. 2007 Jan;81(1):297-305. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    NOK-1 PE


    Fig. 1. Surface staining of CD178 in CD178-transfected L5178Y cells with anti-CD178 (NOK-1) PE.

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