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Rat Monoclonal to CD5 (mouse)

53-7.3 (IgG2a)

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Background

CD5 (T1) is a single-chain transmembrane glycoprotein expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies.

Specificity

The rat monoclonal antibody 53-7.3 recognizes CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B-1 lymphocytes.

Regulatory Status

Immunogen

mouse thymus or spleen cells

Species Reactivity:

  • Mouse

Negative Species:

Applications:

  • Flow Cytometry
  • Immunoprecipitation
  • Immunohistochemistry (paraffin sections)
  • Immunohistochemistry (frozen sections)
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Wang JY, Lee J, Yan M, Rho JH, Roehrl MH: Dermatan sulfate interacts with dead cells and regulates CD5(+) B-cell fate: implications for a key role in autoimmunity. Am J Pathol. 2011 May;178(5):2168-76. [Abstract] [Full Text]
  • *Jeong YI, Hong SH, Cho SH, Lee WJ, Lee SE: Induction of IL-10-producing CD1dhighCD5+ regulatory B cells following Babesia microti-infection. PLoS One. 2012;7(10):e46553. doi: 10.1371/journal.pone.0046553. [Abstract] [Full Text]
  • *Sestero CM, McGuire DJ, De Sarno P, Brantley EC, Soldevila G, Axtell RC, Raman C: CD5-dependent CK2 activation pathway regulates threshold for T cell anergy. J Immunol. 2012 Sep 15;189(6):2918-30 [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    53-7.3 PE


    Fig. 1. Surface staining of murine splenocytes with anti-CD5 (53-7.3) PE.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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