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Mouse IgG2a Isotype Control

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Background

The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-173, generated against an undefined antigen, does not react specifically with mouse, rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.

Specificity

This mouse IgG2a monoclonal antibody (clone MOPC-173) reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Regulatory Status

Immunogen

The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice.

Negative Species:

  • Human
  • Mouse
  • Rat

Applications:

  • Flow Cytometry
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry
  • Immunocytochemistry
  • Control Experiments
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Fougereau M, Bourgois A, de Preval C, Rocca-Serra J, Schiff C: The complete sequence of the murine monoclonal immunoglobulin MOPC 173 (IgG2a): genetic implications. Ann Immunol (Paris). 1976 Sep-Oct;127(5):607-31. [Abstract]
  • *Baumal R, Scharff MD: Immunoglobulin biosynthesis by the MOPC 173 mouse myeloma tumor and a variant spleen clone. J Immunol. 1976 Jan;116(1):65-74. [Abstract]
  • *Gupta V, Gylling A, Alonso JL, Sugimori T, Ianakiev P, Xiong JP, Arnaout MA: The beta-tail domain (betaTD) regulates physiologic ligand binding to integrin CD11b/CD18. Blood. 2007 Apr 15;109(8):3513-20. [Abstract] [Full Text]
  • *Khoddami V, Cairns BR: Transcriptome-wide target profiling of RNA cytosine methyltransferases using the mechanism-based enrichment procedure Aza-IP. Nat Protoc. 2014 Feb;9(2):337-61. [Abstract]
  • *And many other.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    MOPC-173 A7


    Fig. 1. Example of nonspecific mouse IgG2a (MOPC-173) Alexa Fluor® 700 signal on human peripheral blood; surface staining. Upper panel: 3 μg/ml, lower panel: 9 μg/ml.




    MOPC-173 A4


    Fig. 2. Example of nonspecific mouse IgG2a (MOPC-173) Alexa Fluor® 488 signal on human peripheral blood; surface staining, 27 μg/ml.



    MOPC-173 APC


    Fig. 3. Example of nonspecific mouse IgG2a (MOPC-173) APC signal on human peripheral blood; surface staining, 9 μg/ml.



    MOPC-173 PE


    Fig. 4. Example of nonspecific mouse IgG2a (MOPC-173) PE signal on human peripheral blood; surface staining, 9 μg/ml.


    mopc-173 PerCP


    Fig. 5. Example of nonspecific mouse IgG2a (MOPC-173) PerCP signal on human peripheral blood; surface staining, 9 μg/ml.


    11-724 FC


    Fig. 5. Example of nonspecific mouse IgG2a (MOPC-173) purified / GAM-APC signal (red) on human peripheral blood compared with blank (black).

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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