System Certification ISO 9001:2000
Exbio Antibodies CZ / SK eShop
by categories | a-z list | Application type: Flow Cytometry | Immunohistochemistry | Functional Studies

Mouse Monoclonal to CD116

4H1 (IgG1)

Technical Information Request    Inquiry for Bulk Prices

Product No. Form Quantity Stock Price Datasheet Shop
  11-760-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-760-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1P-760-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
  1P-760-T100 PE 100 tests yes choose region PDF datasheetHTML datasheet buy
- In stock! Ready for immediate despatch - Manufacture on order

Background

CD116 (GM-CSF R alpha) is the low affinity receptor for granulocyte-macrophage colony-stimulating factor (GM-CSF). CD116 heterodimerizes with CD131, the common beta chain subunit shared with IL-3 and IL5 receptors, to form the high affinity GM-CSF receptor. CD116 is expressed by myeloid cells including macrophages, neutrophils, eosinophils, dendritic cells, and their precursors, as well as on endothelial cells. It is being used as a specific marker of myeloid leukemias.

Specificity

The mouse monoclonal antibody 4H1 recognizes human CD116, the GM-CSF receptor alpha subunit (approx. 80 kDa) expressed e.g. by neutrophils, eosinophils, monocytes and macrophages.

Regulatory Status

Immunogen

CD116-transfected COS cells

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry (frozen sections)
  • Mass Cytometry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • Stomski FC, Woodcock JM, Zacharakis B, Bagley CJ, Sun Q, Lopez AF: Identification of a Cys motif in the common beta chain of the interleukin 3, granulocyte-macrophage colony-stimulating factor, and interleukin 5 receptors essential for disulfide-linked receptor heterodimerization and activation of all three receptors. J Biol Chem. 1998 Jan 9;273(2):1192-9. [Abstract] [Full Text]
  • *Huntington ND, Legrand N, Alves NL, Jaron B, Weijer K, Plet A, Corcuff E, Mortier E, Jacques Y, Spits H, Di Santo JP: IL-15 trans-presentation promotes human NK cell development and differentiation in vivo. J Exp Med. 2009 Jan 16;206(1):25-34 [Abstract] [Full Text]
  • *Koba C, Haruta M, Matsunaga Y, Matsumura K, Haga E, Sasaki Y, Ikeda T, Takamatsu K, Nishimura Y, Senju S: Therapeutic effect of human iPS-cell-derived myeloid cells expressing IFN-β against peritoneally disseminated cancer in xenograft models. PLoS One. 2013 Jun 24;8(6):e67567 [Abstract] [Full Text]
  • *Schwarzmaier D, Foell D, Weinhage T, Varga G, Däbritz J: Peripheral monocyte functions and activation in patients with quiescent Crohn's disease. PLoS One. 2013 Apr 26;8(4):e62761. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    4H1 purified MC

    Fig. 1. Surface staining (mass cytometry) of human peripheral blood with anti-CD116 (4H1) 153Eu. Gated on singlets.


    4H1 PE

    Fig. 2. Surface staining (flow cytometry) of human peripheral blood with anti-CD116 (4H1) PE.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
    © 2003 EXBIO. All rights reserved.
    terms and conditions | privacy policy