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Rat Monoclonal to CD45R (human, mouse)

RA3-6B2 (IgG2a)

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Product No. Form Quantity Stock Price Datasheet Shop
  11-715-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-715-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  12-715-C100 low endotoxin 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-715-C025 FITC 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1F-715-C100 FITC 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1P-715-C025 PE 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1P-715-C100 PE 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1A-715-C025 APC 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1A-715-C100 APC 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

CD45R, also known as B220, is a receptor-type protein tyrosine phosphatase glycoprotein. It is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45R expression also identifies a subset of murine bone marrow cells able to form osteoclast-like cells.

Specificity

The rat monoclonal antibody RA3-6B2 recognizes an epitope on CD45R, which is expressed at all developmental stages of B cells, including activated B cells, but also on subsets of NK and T cells. T cells detected by this antibody are supposed to be in activated state.

Regulatory Status

Immunogen

Abelson murine leukemia virus-induced pre-B tumor cells

Species Reactivity:

  • Human
  • Mouse
  • Feline (Cat)

Negative Species:

Applications:

  • Flow Cytometry
  • Immunoprecipitation
  • Immunohistochemistry (paraffin sections)
  • Immunohistochemistry (frozen sections)
  • Immunocytochemistry
  • Functional Application
    modulation of B cell responses
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Watanabe Y, Akaike T: Activation signal induces the expression of B cell-specific CD45R epitope (6B2) on murine T cells. Scand J Immunol. 1994 May;39(5):419-25. [Abstract]
  • *Tsurui H, Nishimura H, Hattori S, Hirose S, Okumura K, Shirai T: Seven-color fluorescence imaging of tissue samples based on Fourier spectroscopy and singular value decomposition. J Histochem Cytochem. 2000 May;48(5):653-62. [Abstract]
  • *Rada C, Ehrenstein MR, Neuberger MS, Milstein C: Hot spot focusing of somatic hypermutation in MSH2-deficient mice suggests two stages of mutational targeting. Immunity. 1998 Jul;9(1):135-41. [Abstract]
  • *Rothaeusler K, Baumgarth N: Evaluation of intranuclear BrdU detection procedures for use in multicolor flow cytometry. Cytometry A. 2006 Apr;69(4):249-59. [Abstract] [Full Text]
  • *Montecino-Rodriguez E, Leathers H, Dorshkind K: Identification of a B-1 B cell-specified progenitor. Nat Immunol. 2006 Mar;7(3):293-301. [Abstract]
  • *Cappione AJ, Pugh-Bernard AE, Anolik JH, Sanz I: Lupus IgG VH4.34 antibodies bind to a 220-kDa glycoform of CD45/B220 on the surface of human B lymphocytes. J Immunol. 2004 Apr 1;172(7):4298-307. [Abstract] [Full Text]
  • *Lochhead RB, Sonderegger FL, Ma Y, Brewster JE, Cornwall D, Maylor-Hagen H, Miller JC, Zachary JF, Weis JH, Weis JJ: Endothelial cells and fibroblasts amplify the arthritogenic type I IFN response in murine Lyme disease and are major sources of chemokines in Borrelia burgdorferi-infected joint tissue. J Immunol. 2012 Sep 1;189(5):2488-501 [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    RA3-6B2 APC


    Fig. 1. Surface staining of CD45R in murine splenocytes with anti-CD45R (RA3-6B2) APC.


    RA3-6B2 PE


    Fig. 2. Surface staining of CD45R in murine splenocytes with anti-CD45R (RA3-6B2) PE.


    1F-715


    Fig. 3. Surface staining of CD45R in murine splenocytes with anti-CD45R (RA3-6B2) FITC.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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