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Mouse Monoclonal to CD32 / FcgammaRII

3D3 (IgG1)

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  11-751-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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Background

CD32 (FcgammaRII) is a low affinity receptor for aggregated IgG. It is strongly expressed on monocytes, granulocytes, myeloid and myeloblastic cell lines, and weakly on B cells, CD34+ bone marrow cells, and resting and activated platelets. After binding its ligand, CD32 induces IgG-mediated phagocytosis and oxidative burst in monocytes and neutrophils, whereas in B cells it mediates a negative signal. This polymorphic transmembrane glycoprotein is expressed not only in the activating (CD32a) and inhibitory isoform (CD32b), but also in individual variants with differing avidities for IgG subtypes (e.g. the CD32a131R and CD32a131H allotypes).

Specificity

The mouse monoclonal antibody 3D3 recognizes CD32, a 40 kDa polymorphic transmembrane glycoprotein serving as the low affinity receptor for aggregated IgG. This antibody recognizes CD32 on B cells of all donors, but on platelets, monocytes, and granulocytes of only some donors (131R variant, but not 131H variant).

Regulatory Status

Immunogen

purified glycosylated recombinant human FcgammaRIIa2

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
  • Mass Cytometry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Vely F, Gruel N, Moncuit J, Cochet O, Rouard H, Dare S, Galon J, Sautes C, Fridman WH, Teillaud JL: A new set of monoclonal antibodies against human Fc gamma RII (CD32) and Fc gamma RIII (CD16): characterization and use in various assays. Hybridoma. 1997 Dec;16(6):519-28. [Abstract]
  • *Boruchov AM, Heller G, Veri MC, Bonvini E, Ravetch JV, Young JW: Activating and inhibitory IgG Fc receptors on human DCs mediate opposing functions. J Clin Invest. 2005 Oct;115(10):2914-23. [Abstract] [Full Text]
  • *Dutertre CA, Bonnin-Gliz E, Pulford K, Bourel D, Fridman WH, Teillaud JL: A novel subset of NK cells expressing high levels of inhibitory FcgammaRIIB modulating antibody-dependent function. J Leukoc Biol. 2008 Dec;84(6):1511-20 [Abstract] [Full Text]
  • *Os A, Brgler S, Ribes AP, Funderud A, Wang D, Thompson KM, Tjønnfjord GE, Bogen B, Munthe LA: Chronic lymphocytic leukemia cells are activated and proliferate in response to specific T helper cells. Cell Rep. 2013 Aug 15;4(3):566-77. [Abstract]
  • *Dai X, Jayapal M, Tay HK, Reghunathan R, Lin G, Too CT, Lim YT, Chan SH, Kemeny DM, Floto RA, Smith KG, Melendez AJ, MacAry PA: Differential signal transduction, membrane trafficking, and immune effector functions mediated by FcgammaRI versus FcgammaRIIa. Blood. 2009 Jul 9;114(2):318-27. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    3D3 purified MC


    Fig. 1. Surface staining (mass cytometry) of human peripheral blood with anti-CD32 (3D3) 167Er. Gated on singlets.


    3d3


    Fig. 2. Surface staining of human peripheral blood with anti-human CD32 (3D3) PE.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIOs term and conditions which are available at www.exbio.cz.
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