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Mouse Monoclonal to CD79a

HM47 (IgG1)

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  11-731-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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  1P-731-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
  1P-731-T100 PE 100 tests yes choose region PDF datasheetHTML datasheet buy
  1A-731-T025 APC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1A-731-T100 APC 100 tests yes choose region PDF datasheetHTML datasheet buy
  T9-731-T025 PerCP-Cy™5.5 25 tests yes choose region PDF datasheetHTML datasheet buy
  T9-731-T100 PerCP-Cy™5.5 100 tests yes choose region PDF datasheetHTML datasheet buy
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Background

CD79a (Ig alpha, MB1) forms disulfide-linked heterodimer with CD79b (Ig beta). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.

Specificity

The mouse monoclonal antibody HM47 reacts with intracellular domain of CD79a (Ig alpha), a 40-45 kDa subunit of B cell antigen-specific receptor (BCR) and its early developmental forms.

Regulatory Status

Immunogen

Synthetic peptide corresponding to C terminal amino acids 208-222 of human CD79a

Species Reactivity:

  • Human
  • Non-Human Primates
  • Porcine
  • Mouse
  • Rat
  • Bovine
  • Canine (Dog)
  • Equine (Horse)
  • Guinea pig
  • Rabbit
  • Chicken

Negative Species:

Applications:

  • Flow Cytometry
    Application note: intracellular staining
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry (paraffin sections)
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Mason DY, Cordell JL, Tse AG, van Dongen JJ, van Noesel CJ, Micklem K, Pulford KA, Valensi F, Comans-Bitter WM, Borst J, et al.: The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells. J Immunol. 1991 Dec 1;147(11):2474-82. [Abstract]
  • *Bhargava P, Kallakury BV, Ross JS, Azumi N, Bagg A: CD79a is heterogeneously expressed in neoplastic and normal myeloid precursors and megakaryocytes in an antibody clone-dependent manner. Am J Clin Pathol. 2007 Aug;128(2):306-13. [Abstract] [Full Text]
  • *Rassenti LZ, Kipps TJ: Expression of Ig-beta (CD79b) by chronic lymphocytic leukemia B cells that lack immunoglobulin heavy-chain allelic exclusion. Blood. 2000 Apr 15;95(8):2725-7. [Abstract] [Full Text]
  • *Fernandez NJ, West KH, Jackson ML, Kidney BA: Immunohistochemical and histochemical stains for differentiating canine cutaneous round cell tumors. Vet Pathol. 2005 Jul;42(4):437-45. [Abstract] [Full Text]
  • *Torlakovic E, Torlakovic G: B-cell markers in lymphocyte predominance Hodgkin disease. Arch Pathol Lab Med. 2002 Jul;126(7):862-3. [Abstract] [Full Text]
  • *Zhao XF, Hassan A, Perry A, Ning Y, Stass SA, Dehner LP: C-MYC rearrangements are frequent in aggressive mature B-Cell lymphoma with atypical morphology. Int J Clin Exp Pathol. 2008 Jan 1;1(1):65-74. [Abstract] [Full Text]
  • *Islas-Ohlmayer M, Padgett-Thomas A, Domiati-Saad R, Melkus MW, Cravens PD, Martin Mdel P, Netto G, Garcia JV: Experimental infection of NOD/SCID mice reconstituted with human CD34+ cells with Epstein-Barr virus. J Virol. 2004 Dec;78(24):13891-900. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    HM47 PE


    Fig. 1. Surface staining of CD79a in human peripheral blood with anti-CD79a (HM47) PE.


    HM47 PerCP-Cy5.5


    Fig. 2. Surface staining of CD79a in human peripheral blood with anti-CD79a (HM47) PerCP-CyTM5.5.



    HM47 APC


    Fig. 2. Surface staining of CD79a in human peripheral blood with anti-CD79a (HM47) APC.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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