categories: CD and Related Antigens (Human), CD Antigens & MHC (Veterinary), CD and Related Antigens & MHC (Rodent)

Mouse Monoclonal to CD79a

HM47 (IgG1)

Product No.	Form	Quantity	Price
11-731-C025	purified	0.025 mg	choose region
11-731-C100	purified	0.1 mg	choose region
1P-731-T025	PE	25 tests	choose region
1P-731-T100	PE	100 tests	choose region
1A-731-T025	APC	25 tests	choose region
1A-731-T100	APC	100 tests	choose region
T9-731-T025	PerCP-Cy™5.5	25 tests	choose region
T9-731-T100	PerCP-Cy™5.5	100 tests	choose region

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Background

CD79a (Ig alpha, MB1) forms disulfide-linked heterodimer with CD79b (Ig beta). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.

Entrez Gene (human): 19q13.2
Entrez Gene (bovine): 18
Entrez Gene (mouse): 7 5.5 cM

Specificity

The mouse monoclonal antibody HM47 reacts with intracellular domain of CD79a (Ig alpha), a 40-45 kDa subunit of B cell antigen-specific receptor (BCR) and its early developmental forms.

Regulatory Status

RUO

Immunogen

Synthetic peptide corresponding to C terminal amino acids 208-222 of human CD79a

Species Reactivity: Human Non-Human Primates Porcine Mouse Rat Bovine Canine (Dog) Equine (Horse) Guinea pig Rabbit Chicken Negative Species:	Applications: Flow Cytometry Application note: intracellular staining Immunoprecipitation Western Blotting Immunohistochemistry (paraffin sections) Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	Product Specific References Mason DY, Cordell JL, Tse AG, van Dongen JJ, van Noesel CJ, Micklem K, Pulford KA, Valensi F, Comans-Bitter WM, Borst J, et al.: The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells. J Immunol. 1991 Dec 1;147(11):2474-82. [Abstract] Bhargava P, Kallakury BV, Ross JS, Azumi N, Bagg A: CD79a is heterogeneously expressed in neoplastic and normal myeloid precursors and megakaryocytes in an antibody clone-dependent manner. Am J Clin Pathol. 2007 Aug;128(2):306-13. [Abstract] [Full Text] Rassenti LZ, Kipps TJ: Expression of Ig-beta (CD79b) by chronic lymphocytic leukemia B cells that lack immunoglobulin heavy-chain allelic exclusion. Blood. 2000 Apr 15;95(8):2725-7. [Abstract] [Full Text] Fernandez NJ, West KH, Jackson ML, Kidney BA: Immunohistochemical and histochemical stains for differentiating canine cutaneous round cell tumors. Vet Pathol. 2005 Jul;42(4):437-45. [Abstract] [Full Text] Torlakovic E, Torlakovic G: B-cell markers in lymphocyte predominance Hodgkin disease. Arch Pathol Lab Med. 2002 Jul;126(7):862-3. [Abstract] [Full Text] Zhao XF, Hassan A, Perry A, Ning Y, Stass SA, Dehner LP: C-MYC rearrangements are frequent in aggressive mature B-Cell lymphoma with atypical morphology. Int J Clin Exp Pathol. 2008 Jan 1;1(1):65-74. [Abstract] [Full Text] *Islas-Ohlmayer M, Padgett-Thomas A, Domiati-Saad R, Melkus MW, Cravens PD, Martin Mdel P, Netto G, Garcia JV: Experimental infection of NOD/SCID mice reconstituted with human CD34+ cells with Epstein-Barr virus. J Virol. 2004 Dec;78(24):13891-900. [Abstract] [Full Text]

Species Reactivity:

Human
Non-Human Primates
Porcine
Mouse
Rat
Bovine
Canine (Dog)
Equine (Horse)
Guinea pig
Rabbit
Chicken

Negative Species:

Applications:

Flow Cytometry
Application note: intracellular staining
Immunoprecipitation
Western Blotting
Immunohistochemistry (paraffin sections)

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

*Mason DY, Cordell JL, Tse AG, van Dongen JJ, van Noesel CJ, Micklem K, Pulford KA, Valensi F, Comans-Bitter WM, Borst J, et al.: The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells. J Immunol. 1991 Dec 1;147(11):2474-82. [Abstract]

*Bhargava P, Kallakury BV, Ross JS, Azumi N, Bagg A: CD79a is heterogeneously expressed in neoplastic and normal myeloid precursors and megakaryocytes in an antibody clone-dependent manner. Am J Clin Pathol. 2007 Aug;128(2):306-13. [Abstract] [Full Text]

*Rassenti LZ, Kipps TJ: Expression of Ig-beta (CD79b) by chronic lymphocytic leukemia B cells that lack immunoglobulin heavy-chain allelic exclusion. Blood. 2000 Apr 15;95(8):2725-7. [Abstract] [Full Text]

*Fernandez NJ, West KH, Jackson ML, Kidney BA: Immunohistochemical and histochemical stains for differentiating canine cutaneous round cell tumors. Vet Pathol. 2005 Jul;42(4):437-45. [Abstract] [Full Text]

*Torlakovic E, Torlakovic G: B-cell markers in lymphocyte predominance Hodgkin disease. Arch Pathol Lab Med. 2002 Jul;126(7):862-3. [Abstract] [Full Text]

*Zhao XF, Hassan A, Perry A, Ning Y, Stass SA, Dehner LP: C-MYC rearrangements are frequent in aggressive mature B-Cell lymphoma with atypical morphology. Int J Clin Exp Pathol. 2008 Jan 1;1(1):65-74. [Abstract] [Full Text]

*Islas-Ohlmayer M, Padgett-Thomas A, Domiati-Saad R, Melkus MW, Cravens PD, Martin Mdel P, Netto G, Garcia JV: Experimental infection of NOD/SCID mice reconstituted with human CD34+ cells with Epstein-Barr virus. J Virol. 2004 Dec;78(24):13891-900. [Abstract] [Full Text]

For research use only. Not for drug, diagnostic or other use.

Example Data

Fig. 1. Intracellular staining of CD79a in human peripheral blood with anti-CD79a (HM47) PE.

Fig. 2. Intracellular staining of CD79a in human peripheral blood with anti-CD79a (HM47) PerCP-Cy^TM5.5.

Fig. 2. Intracellular staining of CD79a in human peripheral blood with anti-CD79a (HM47) APC.

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