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Mouse Monoclonal to NCK1

EM-06 (IgG1)

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NCK1 (NCK alpha) is a cytoplasmic adaptor protein that plays a universal role in coordinating the signaling networks critical for organizing the actin cytoskeleton, cell movement, or axon guidance, connecting transmembrane receptors to multiple intracellular signaling pathways. It contains one SH2 domain, through which NCK1 binds to phosphorylated domains of transmembrane signaling moleculs or certain adaptor proteins, and three SH3 domains for binding proline-rich sequences of other molecules involved in the process of nucleation and polymerization of the actin cytoskeleton.


The mouse monoclonal antibody EM-06 recognizes NCK1 (NCK alpha), an ubiquitously expressed cytoplasmic SH2/SH3 adaptor protein important for organization of actin cytoskeleton structures.

Regulatory Status


Purified recombinant second SH3 domain of human Nck1 fused to GST

Species Reactivity:

  • Human
  • Mouse

Negative Species:


  • Western Blotting
    Recommended dilution: 2 μg/ml
    Positive control: Jurkat cell lysate
  • Immunocytochemistry
    Recommended dilution: 2 μg/ml
    Positive control: Human NCK1-transfected COS-7 cells
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Borroto A, Arellano I, Dopfer EP, Prouza M, Suchánek M, Fuentes M, Orfao A, Schamel WW, Alarcón B: Nck recruitment to the TCR required for ZAP70 activation during thymic development. J Immunol. 2013 Feb 1;190(3):1103-12. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    Fig. 1.

    Fig. 1. Western blotting analysis of NCK1 in whole cell lysate of mouse lymph node lymphocytes using the antibody EM-06.

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