Mouse Monoclonal to CD3 zeta (Phospho-Tyr153)
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complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
The mouse monoclonal antibody EM-17 recognizes phosphorylated tyrosine 153 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.
A phospho specific peptide corresponding to the amino acids surrounding tyrosine 153 of mouse CD3 zeta linked to KLH
- Flow Cytometry
Recommended dilution: 2 - 10 µg/ml
Positive control: Jurkat cells treated with pervanadate
- Western Blotting
Recommended dilution: 2 - 5 µg/ml
Positive control: Jurkat cells lysate treated with pervanadate; Splenocyte lysate of Balb/c or F1 mouse treated with pervanadate
Application note: Non-reducing conditions recommended
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
For research use only. Not for drug, diagnostic or other use.
Reactivity of the monoclonal antibodies EM-26
(anti-CD3 zeta phospho-Tyr72) and EM-17
(anti-CD3 zeta phospho-Tyr153)
with phosphorylated particular human CD3 zeta mutants. The Y1F and Y6F
mutatants lack phosphotyrosine 72 and 153, respectively.