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Mouse Monoclonal to CLIC5a

CLIC5-02 (IgG2b)

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CLIC5a belongs to the family of chloride intracellular channel (CLIC) proteins, all sharing a highly conserved C terminus and variable N terminus. Human CLIC5 is transcribed in two isoforms, 32 kDa CLIC5a (251 amino acids) and 49 kDa CLIC5b (410 amino acids). These proteins exist in a soluble form and their function as ion channels in vitro has not been fully confirmed in vivo. CLIC5a is a component of complexes of actin, ezrin, and several other actin-associated proteins and is important for functionality of actin-based structures.


Mouse monoclonal antibody CLIC5-02 recognizes CLIC5a, a 32 kDa protein which is component of actin complexes. Crossreactivity with CLIC5b was not determined.

Regulatory Status


KLH-conjugated peptide corresponding to the amino acids 160-173 of human CLIC5a

Species Reactivity:

  • Human

Negative Species:


  • Flow Cytometry
  • Western Blotting
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Wegner B, Al-Momany A, Kulak SC, Kozlowski K, Obeidat M, Jahroudi N, Paes J, Berryman M, Ballermann BJ: CLIC5A, a component of the ezrin-podocalyxin complex in glomeruli, is a determinant of podocyte integrity. Am J Physiol Renal Physiol. 2010 Jun;298(6):F1492-503. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    CLIC5a WB
    Fig. 1.

    Fig. 1. Western blotting analysis of CLIC5a in HEK293-CLIC5a transfectants (A) and HEK293 cells (B) using mouse monoclonal antibody (clone CLIC5-02).

    CLIC5 PE

    Fig. 2. Flow cytometry analysis (intracellular staining) of CLIC5a on CLIC5a transfected HEK293 cells using mouse monoclonal antibody (CLIC5-02) PE.

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