categories: Cytoskeleton: Actin System (Human)

Mouse Monoclonal to CLIC5a

CLIC5-02 (IgG2b)

Product No.	Form	Quantity	Price
11-701-C025	purified	0.025 mg	choose region
11-701-C100	purified	0.1 mg	choose region
1P-701-C025	PE	0.025 mg	choose region
1P-701-C100	PE	0.1 mg	choose region

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Background

CLIC5a belongs to the family of chloride intracellular channel (CLIC) proteins, all sharing a highly conserved C terminus and variable N terminus. Human CLIC5 is transcribed in two isoforms, 32 kDa CLIC5a (251 amino acids) and 49 kDa CLIC5b (410 amino acids). These proteins exist in a soluble form and their function as ion channels in vitro has not been fully confirmed in vivo. CLIC5a is a component of complexes of actin, ezrin, and several other actin-associated proteins and is important for functionality of actin-based structures.

Entrez Gene: 6p12.3

Specificity

Mouse monoclonal antibody CLIC5-02 recognizes CLIC5a, a 32 kDa protein which is component of actin complexes. Crossreactivity with CLIC5b was not determined.

Regulatory Status

RUO

Immunogen

KLH-conjugated peptide corresponding to the amino acids 160-173 of human CLIC5a

Species Reactivity: Human Negative Species:	Applications: Flow Cytometry Western Blotting Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references *Wegner B, Al-Momany A, Kulak SC, Kozlowski K, Obeidat M, Jahroudi N, Paes J, Berryman M, Ballermann BJ: CLIC5A, a component of the ezrin-podocalyxin complex in glomeruli, is a determinant of podocyte integrity. Am J Physiol Renal Physiol. 2010 Jun;298(6):F1492-503. [Abstract] [Full Text]

Species Reactivity:

Human

Negative Species:

Applications:

Flow Cytometry
Western Blotting

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Wegner B, Al-Momany A, Kulak SC, Kozlowski K, Obeidat M, Jahroudi N, Paes J, Berryman M, Ballermann BJ: CLIC5A, a component of the ezrin-podocalyxin complex in glomeruli, is a determinant of podocyte integrity. Am J Physiol Renal Physiol. 2010 Jun;298(6):F1492-503. [Abstract] [Full Text]

For research use only. Not for drug, diagnostic or other use.

Example Data

Fig. 1.

Fig. 1. Western blotting analysis of CLIC5a in HEK293-CLIC5a transfectants (A) and HEK293 cells (B) using mouse monoclonal antibody (clone CLIC5-02).

Fig. 2. Flow cytometry analysis (intracellular staining) of CLIC5a on CLIC5a transfected HEK293 cells using mouse monoclonal antibody (CLIC5-02) PE.

Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.

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