Mouse Monoclonal to CLIC5a
CLIC5-02 (IgG2b)
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Background
CLIC5a belongs to the family of chloride intracellular channel (CLIC) proteins, all sharing a highly conserved C terminus and variable N terminus. Human CLIC5 is transcribed in two isoforms, 32 kDa CLIC5a (251 amino acids) and 49 kDa CLIC5b (410 amino acids). These proteins exist in a soluble form and their function as ion channels in vitro has not been fully confirmed in vivo. CLIC5a is a component of complexes of actin, ezrin, and several other actin-associated proteins and is important for functionality of actin-based structures.
Specificity
Mouse monoclonal antibody CLIC5-02 recognizes CLIC5a, a 32 kDa protein which is component of actin complexes. Crossreactivity with CLIC5b was not determined.
Regulatory Status
Immunogen
KLH-conjugated peptide corresponding to the amino acids 160-173 of human CLIC5a
Species Reactivity:
Negative Species:
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Applications:
- Flow Cytometry
- Western Blotting
Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
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General references
*Wegner B, Al-Momany A, Kulak SC, Kozlowski K, Obeidat M, Jahroudi N, Paes J, Berryman M, Ballermann BJ: CLIC5A, a component of the ezrin-podocalyxin complex in glomeruli, is a determinant of podocyte integrity. Am J Physiol Renal Physiol. 2010 Jun;298(6):F1492-503. [Abstract] [Full Text]
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For research use only. Not for drug, diagnostic or other use.
Example Data

Fig. 1.
Fig. 1. Western
blotting analysis of CLIC5a in HEK293-CLIC5a transfectants (A) and
HEK293 cells (B) using mouse monoclonal antibody (clone
CLIC5-02).
Fig. 2. Flow cytometry analysis (intracellular staining) of CLIC5a on CLIC5a transfected HEK293 cells using mouse monoclonal antibody (
CLIC5-02) PE.