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Mouse Monoclonal to LARGE1

LARGE-02 (IgG2b)

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Background

LARGE1 serves as a glycosyltransferase which participates in glycosylation of the muscle membrane protein alpha-dystroglycan. Mutations of LARGE1 lead to hypoglycosylation of alpha-dystroglycan and cause congenital muscular dystrophy (MDC1D) associated with severe mental retardation. Altered alpha-dystroglycan glycosylation may also play a role in cancer, as hypoglycosylation of the protein and loss of laminin binding have been demonstrated in invasive carcinoma cells.

Specificity

The mouse monoclonal antibody LARGE-02 recognizes human LARGE1, a glycosyltransferase localizing mainly to the Golgi apparatus. Crossreactivity with LARGE2 was not determined.

Regulatory Status

Immunogen

Recombinant fragment of human LARGE1 (amino acids 35-142)

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
  • Western Blotting
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Grewal PK, McLaughlan JM, Moore CJ, Browning CA, Hewitt JE: Characterization of the LARGE family of putative glycosyltransferases associated with dystroglycanopathies. Glycobiology. 2005 Oct;15(10):912-23. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    LARGE WB
    Fig. 1.


    Fig. 1. Western blotting analysis of LARGE1 in HEK293-LARGE1 transfectants (A) and HEK293 cells (B) using mouse monoclonal antibody (clone LARGE-02).


    LARGE-02 PE


    Fig. 2. Flow cytometry analysis of LARGE1 in HEK293-LARGE1 transfectants using mouse monoclonal antibody (clone LARGE-02) PE.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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