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Mouse Monoclonal to IFN-gamma

4S.B3 (IgG1)

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Background

The Interferon gamma (IFN-gamma; 16-25 kDa) is an important regulator of the immune response, produced in activated Th1 cells and NK cells, particularly in response to IL-2, TNF-alpha and IL-12; its production is suppressed by IL-4, IL-10, and TGF-beta. The producing of IFN-gamma is activated by specific antigens or mitogens through the T cell antigen receptor. IFN-gamma polypeptide forms: 40-60 kDa forms are observable under non-denaturing conditions as dimers and trimers; 20 kDa and 25 kDa forms exist due to variable glycosylation. IFN-gamma belongs to the type II interferons, also called immune IFN.
IFN-gamma shows antiviral activity and has important immunoregulatory functions. It is a potent activator of macrophages and had antiproliferative effects on transformed cells. IFN-gamma plays an important role in regulating B cell differentiation by simultaneously stimulating class switch recombination to the IgG3 and IgG2a isotypes while represing class switch recombination to the IgE and IgG1 isotypes. It also appears to promote antigen presentation by B cells through its effects on MHC. Binding of IFN-gamma to its receptor increases the expression of class I MHC on all somatic cells. It also enhances the expression of class II MHC on antigen-presenting cells. IFN-gamma is the major means by which T cells activate macrophages, increasing their ability to kill bacteria, parasites, and tumours. The activation of macrophages by IFN-gamma is essential for the elimination of bacteria that replicate within the phagosomes of macrophages (f.e. Mycobacteria and Listeria monocytogenes). IFN-gamma can potentiate the high antiviral and antitumor effects of the type I interferons (IFN-alpha, IFN-beta). IFN-gamma may also activate neutrophils and NK cells.

Specificity

The mouse monoclonal antibody 4S.B3 recognizes IFN-gamma, a 16-25 kDa cytokine produced by activated Th1 cells and NK cells. Binds both glycosylated and non-glycosylated protein.

Regulatory Status

Immunogen

Interferon gamma derived from human leukocytes

Species Reactivity:

  • Human
  • Non-Human Primates

Negative Species:

Applications:

  • Flow Cytometry
    Application note:intracellular staining
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry (paraffin sections)
  • Immunocytochemistry
  • ELISA
    is being used as detection antibody in combination with capture antibody NIB42
  • RIA
  • Functional Application
    Neutralization (weak)
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Meager A, Parti S, Barwick S, Spragg J, O'Hagan K: Detection of hybridomas secreting monoclonal antibodies to human gamma interferon using a rapid screening technique and specificity of certain monoclonal antibodies to gamma interferon. J Interferon Res. 1984 Fall;4(4):619-25. [Abstract]
  • *Caulfield JJ, Fernandez MH, Sousa AR, Lane SJ, Lee TH, Hawrylowicz CM: Regulation of major histocompatibility complex class II antigens on human alveolar macrophages by granulocyte-macrophage colony-stimulating factor in the presence of glucocorticoids. Immunology. 1999 Sep;98(1):104-10. [Abstract] [Full Text]
  • *Brattig NW, Lepping B, Timmann C, Büttner DW, Marfo Y, Hamelmann C, Horstmann RD: Onchocerca volvulus-exposed persons fail to produce interferon-gamma in response to O. volvulus antigen but mount proliferative responses with interleukin-5 and IL-13 production that decrease with increasing microfilarial density. J Infect Dis. 2002 Apr 15;185(8):1148-54. [Abstract] [Full Text]
  • *Janke M, Witsch EJ, Mages HW, Hutloff A, Kroczek RA: Eminent role of ICOS costimulation for T cells interacting with plasmacytoid dendritic cells. Immunology. 2006 Jul;118(3):353-60. [Abstract] [Full Text]
  • *David A. Kosub, Ginger Lehrman, Jeffrey M. Milush, Dejiang Zhou, Elizabeth Chacko, Amanda Leone, Shari Gordon, Guido Silvestri, James G. Else, Philip Keiser, Mamta K. Jain, Donald L. Sodora: Gamma/Delta T-Cell Functional Responses Differ after Pathogenic Human Immunodeficiency Virus and Nonpathogenic Simian Immunodeficiency Virus Infections. J Virol. 2008 February; 82(3): 1155–1165. [Abstract]
  • *Erik L. Brincks, Tamara A. Kucaba, Kevin L. Legge, Thomas S. Griffith: Influenza-induced expression of functional TNF-related apoptosis-inducing ligand (TRAIL) on human PBMC. Hum Immunol. 2008 October; 69(10): 634–646. [Abstract]
  • *Coles AJ, Wing M, Smith S, Coraddu F, Greer S, Taylor C, Weetman A, Hale G, Chatterjee VK, Waldmann H, Compston A: Pulsed monoclonal antibody treatment and autoimmune thyroid disease in multiple sclerosis. Lancet. 1999 Nov 13;354(9191):1691-5. [Abstract]
  • *And many other.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    4S.B3 PerCP-Cy5.5


    Fig. 1. Intracellular staining of IFN gamma in PHA-activated human PBMC with anti-IFN gamma (4S.B3) PerCP-CyTM5.5.



    4S-B3 PE-Cy7


    Fig. 2. Intracellular staining of IFN gamma in PHA-activated human PBMC with anti-IFN gamma (4S.B3) PE-CyTM7.


    4s-b3 PE


    Fig. 3. Intracellular staining of IFN gamma in PHA-activated human PBMC with anti-IFN gamma (4S.B3) PE.


    4s-b3 APC


    Fig. 4. Intracellular staining of IFN gamma in PHA-activated human PBMC with anti-IFN gamma (4S.B3) APC.


    4S-B3 FITC


    Fig. 5. Intracellular staining of IFN gamma in PHA-activated human PBMC with anti-IFN gamma (4S.B3) FITC.

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