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Mouse Monoclonal to SCIMP

NVL-07 (IgG2a)

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Product No. Form Quantity Stock Price Datasheet Shop
  11-704-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-704-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

SCIMP (SLP adaptor and Csk interacting membrane protein), also known as Nvl, is a palmitoylated transmembrane adaptor protein expressed in professional antigen presenting cells, most prominently in the lymph nodes and spleen. It is associated with tetraspanin-enriched microdomains (together with MHC II). There is a close relationship between SCIMP and tyrosinkinase Lyn, which is constitutively bound to it by its SH3 domain. After MHC II-mediated stimulation in the immunological synapse SCIMP becomes phosphorylated at several tyrosine residues and provides docking sites for Grb2 and SLP65 or SLP76 adaptors transducing the signal downstream, as well as for the kinase Csk with modulatory roles.

Specificity

The mouse monoclonal antibody NVL-07 recognizes intracellular part of human transmembrane adaptor SCIMP. This protein of 17 kDa predicted Mw migrates as a 22 kDa band on SDS PAGE.

Regulatory Status

Immunogen

Recombinant intracellular part of human SCIMP

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
    Application note:intracellular staining
  • Immunoprecipitation
  • Western Blotting
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Draber P, Vonkova I, Stepanek O, Hrdinka M, Kucova M, Skopcova T, Otahal P, Angelisova P, Horejsi V, Yeung M, Weiss A, Brdicka T: SCIMP, a transmembrane adaptor protein involved in major histocompatibility complex class II signaling. Mol Cell Biol. 2011 Nov;31(22):4550-62. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    NVL FC


    Fig. 1. Intracellular staining of SCIMP in a population of HEK-293T-SCIMP transfectants using monoclonal antibody (clone NVL-07, purified).The staining pattern reflects heterogeneity in the cell population regarding transfection efficiency.

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