System Certification ISO 9001:2000
Exbio Antibodies CZ / SK eShop
by categories | a-z list | Application type: Flow Cytometry | Immunohistochemistry | Functional Studies

Mouse Monoclonal to CD195 / CCR5

T21/8 (IgG1)

Technical Information Request    Inquiry for Bulk Prices

Product No. Form Quantity Stock Price Datasheet Shop
  11-707-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-707-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1P-707-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
  1P-707-T100 PE 100 tests yes choose region PDF datasheetHTML datasheet buy
  1A-707-T025 APC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1A-707-T100 APC 100 tests yes choose region PDF datasheetHTML datasheet buy
- In stock! Ready for immediate despatch - Manufacture on order

Background

CD195 / CCR5 (also known as CKR-5) is a receptor for inflammatory CC-chemokines (characterized by a pair of adjacent cysteine residues), such as MIP-1 alpha, MIP-1 beta, or RANTES. It is a G protein-associated seven-pass transmembrane protein expressed on resting T cells with memory/effector phenotype, monocytes, macrophages and immature dendritic cells. This chemokine receptor regulates the activation and directed migration of leukocytes. Importantly, along with CD4, CD195 / CCR5 functions as a major receptor for HIV. Their ligand is the viral glycoprotein gp120.

Specificity

The mouse monoclonal antibody T21/8 recognizes the N-teminus of CD195, an approximately 45 kDa G-protein coupled receptor 1 family protein expressed on resting T cells, monocytes, macrophages, and immature dendritic cells.

Regulatory Status

Immunogen

CCR5 peptide (Met1-Lys22) KLH conjugate

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
  • Immunoprecipitation
  • Western Blotting
    Application note:do not boil cell lysates prior to SDS-PAGE
  • Immunocytochemistry
  • ELISA
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Pollok-Kopp B, Schwarze K, Baradari VK, Oppermann M: Analysis of ligand-stimulated CC chemokine receptor 5 (CCR5) phosphorylation in intact cells using phosphosite-specific antibodies. J Biol Chem. 2003 Jan 24;278(4):2190-8. [Abstract]
  • *Hüttenrauch F, Pollok-Kopp B, Oppermann M: G protein-coupled receptor kinases promote phosphorylation and beta-arrestin-mediated internalization of CCR5 homo- and hetero-oligomers. J Biol Chem. 2005 Nov 11;280(45):37503-15. [Abstract] [Full Text]
  • *Monde K, Maeda Y, Tanaka Y, Harada S, Yusa K: Gp120 V3-dependent impairment of R5 HIV-1 infectivity due to virion-incorporated CCR5. J Biol Chem. 2007 Dec 21;282(51):36923-32. [Abstract] [Full Text]
  • *Mascalchi P, Lamort AS, Salomé L, Dumas F: Single Particle Tracking reveals two distinct environments for CD4 receptors at the surface of living T lymphocytes. Biochem Biophys Res Commun. 2012 Jan 6;417(1):409-13 [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    T21-8 APC


    Fig. 1. Surface staining of CD195 in human peripheral blood with anti-CD195 (T21/8) APC.


    T21-8 PE


    Fig. 2. Surface staining of CD195 in human peripheral blood with anti-CD195 (T21/8) PE.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
    © 2003 EXBIO. All rights reserved.
    terms and conditions | privacy policy