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Mouse Monoclonal to CD35 / CR1

E11 (IgG1)

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  11-703-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-703-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-703-T025 FITC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1F-703-T100 FITC 100 tests yes choose region PDF datasheetHTML datasheet buy
  1P-703-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
  1P-703-T100 PE 100 tests yes choose region PDF datasheetHTML datasheet buy
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Background

CD35 (complement receptor 1, CR1) is a monomeric multiple modular cell surface glycoprotein which serves as receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. It is expressed mainly on the surface of granulocytes, monocytes, erythrocytes, B cells and folicular dendritic cells. Besides its role in complement cascade, CD35 is involved in blocking BCR-induced proliferation and the differentiation of B cells to plasmablasts and their Ig production.

Specificity

The mouse monoclonal antibody E11 recognizes CD35 (CR1), a type I glycoprotein expressed on granulocytes, monocytes, B cells, folicular dendritic cells, erythrocytes, NK and T cell subsets, as well as e.g. on glomerulal podocytes.
HLDA III; WS Code 204

Regulatory Status

Immunogen

Acute monocytic leukemia cells and normal blood monocytes

Species Reactivity:

  • Human
  • Non-Human Primates

Negative Species:

Applications:

  • Flow Cytometry
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry (paraffin sections)
    Application note:heat mediated antigen retrieval
  • Immunohistochemistry (frozen sections)
    Application note:acetone fixation
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Kremlitzka M, Polgr A, Flp L, Kiss E, Por G, Erdei A: Complement receptor type 1 (CR1, CD35) is a potent inhibitor of B-cell functions in rheumatoid arthritis patients. Int Immunol. 2012 Sep 7. [Epub ahead of print] [Abstract]
  • *Nielsen CH, Pedersen ML, Marquart HV, Prodinger WM, Leslie RG: The role of complement receptors type 1 (CR1, CD35) and 2 (CR2, CD21) in promoting C3 fragment deposition and membrane attack complex formation on normal peripheral human B cells. Eur J Immunol. 2002 May;32(5):1359-67. [Abstract]
  • Product Specific References

  • *Leukocyte Typing III., McMichael M.J. et al. (Eds.), Oxford University Press (1987); p.611.
  • *Hogg N, Ross GD, Jones DB, Slusarenko M, Walport MJ, Lachmann PJ: Identification of an anti-monocyte monoclonal antibody that is specific for membrane complement receptor type one (CR1). Eur J Immunol. 1984 Mar;14(3):236-43. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    E11 PE


    Fig. 1. Surface staining of CD35 in human peripheral blood with anti-CD35 (E11) PE.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIOs term and conditions which are available at www.exbio.cz.
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