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Mouse Monoclonal to CD123

6H6 (IgG1)

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Background

CD123 is the alpha chain of interleukin 3 receptor (IL-3R alpha). This subunit heterodimerizes with the interleukin 3 receptor beta chain (CD131), which is shared with other receptors. CD123 interacts with IL-3 specifically, but with low affinity, and association with the beta subunit confers high affinity binding to the receptor heterodimer. Both chains are required for signaling, but receptor activation and signal transduction depend on IL-3 binding to CD123 as the initial step.

Specificity

The mouse monoclonal antibody 6H6 recognizes CD123 (interleukin 3 receptor alpha), a 60-70 kDa transmembrane protein expressed by myeloid precursors, megakaryocytes, macrophages, dendritic cells, mast cells, basophils, and some B cells. This antibody does not inhibit IL-3 binding to its receptor.

Regulatory Status

Immunogen

IL3 receptor alpha chain expressed on the surface of transiently transfected COS cells

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry (frozen sections)
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Sun Q, Woodcock JM, Rapoport A, Stomski FC, Korpelainen EI, Bagley CJ, Goodall GJ, Smith WB, Gamble JR, Vadas MA, Lopez AF: Monoclonal antibody 7G3 recognizes the N-terminal domain of the human interleukin-3 (IL-3) receptor alpha-chain and functions as a specific IL-3 receptor antagonist. Blood. 1996 Jan 1;87(1):83-92. [Abstract] [Full Text]
  • *Martín-Gayo E, Sierra-Filardi E, Corbí AL, Toribio ML: Plasmacytoid dendritic cells resident in human thymus drive natural Treg cell development. Blood. 2010 Jul 1;115(26):5366-75. [Abstract] [Full Text]
  • *Herling M, Teitell MA, Shen RR, Medeiros LJ, Jones D: TCL1 expression in plasmacytoid dendritic cells (DC2s) and the related CD4+ CD56+ blastic tumors of skin. Blood. 2003 Jun 15;101(12):5007-9. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    6H6 PerCP-Cy5-5


    Fig. 1. Surface staining of human peripheral blood with anti-CD123 (6H6) PerCP-CyTM5.5.


    6H6 PE


    Fig. 2. Surface staining of human peripheral blood with anti-CD123 (6H6) PE.



    6H6 PE-Cy7


    Fig. 3. Surface staining of human peripheral blood with anti-CD123 (6H6) PE-CyTM7.


    6H6 PE-Cy5


    Fig. 4. Surface staining of human peripheral blood with anti-CD123 (6H6) PE-CyTM5.



    6H6 APC


    Fig. 5. Surface staining of human peripheral blood with anti-CD123 (6H6) APC.



    1B-700


    Fig. 6. Surface staining of human peripheral blood with anti-CD123 (6H6) biotin.

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