Mouse Monoclonal to PCLO
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(piccolo, also known as aczonin) is a large (more than 400 kDa) multidomain protein of the presynaptic cytomatrix in neurons, that is present in all vertebrate synapses, but is absent from invertebrates. It contains zinc finger and coiled-coil sequences, as well as N-terminal glutamine-rich sequence, and C-terminal PDZ domain followed by two C2 domains (C2A and C2B). In vitro binding and transfection experiments suggested that PCLO binds to multiple proteins including profilin and L-type calcium channels. It is involved in neurotransmitter release and insulin secretion.
The mouse monoclonal antibody PCLO-01 recognizes PCLO (Piccolo), a more than 400 kDa multidomain protein expressed mainly in the presynaptic cytomatrix of the neurons.
Human recombinant PCLO protein
- Flow Cytometry
- Western Blotting
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
*Dresbach T, Torres V, Wittenmayer N, Altrock WD, Zamorano P, Zuschratter W, Nawrotzki R, Ziv NE, Garner CC, Gundelfinger ED: Assembly of active zone precursor vesicles: obligatory trafficking of presynaptic cytomatrix proteins Bassoon and Piccolo via a trans-Golgi compartment. J Biol Chem. 2006 Mar 3;281(9):6038-47. [Abstract] [Full Text]
*Mukherjee K, Yang X, Gerber SH, Kwon HB, Ho A, Castillo PE, Liu X, Südhof TC: Piccolo and bassoon maintain synaptic vesicle clustering without directly participating in vesicle exocytosis. Proc Natl Acad Sci U S A. 2010 Apr 6;107(14):6504-9. [Abstract] [Full Text]
For research use only. Not for drug, diagnostic or other use.
Fig. 1: Western blot of human REH erythroid cell line
lysate (1% laurylmaltoside); non-reduced sample, immunostained by mAb
PCLO-01 and goat anti-mouse IgG (H+L)-HRP conjugate. The zone evidently corresponds to a small
splice variant of the PCLO protein present in this type of cell (see Uniprot
database, Genome Res. 14:2121-2127(2004)); the major form (over 500 kDa)
expressed in neurons is too large for analysis by Western blotting.
Fig. 2: Flow cytometry analysis (intracellular staining) of PCLO in human peripheral blood using mouse monoclonal (PCLO-01) PE.