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Mouse Monoclonal to Drebrin

DBN-N-03 (IgG2b)

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  11-694-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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Background

Drebrin is an actin-binding protein, which is expressed mainly in neurons and plays important role in their morphogenesis. The highest level of its expression is in developing brain. Both in neurons and non-neuronal cells drebrin acts as a key regulator of actin cytoskeleton remodelling, affecting especially intercellular junctions, such as dendritic spines of neurons or the immune synapses of T cells. Decrease of drebrin amount in the brain seems to be associated with Alzheimer's disease and Down syndrome, and in case of B-cell precursor acute lymphoblastic leukemia (BCP-ALL) lower drebrin expression correlates with higher risk of relapse.

Specificity

The mouse monoclonal antibody DBN-N-03 recognizes drebrin, an approximately 100-125 kDa regulator of actin cytoskeleton.

Regulatory Status

Immunogen

Bacterially expressed N-terminal fragment of recombinant human drebrin (aa 1-326)

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
  • Western Blotting
    Positive control:REH, Nalm-6, HEK293 cells
  • Mass Cytometry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Ishikawa R, Hayashi K, Shirao T, Xue Y, Takagi T, Sasaki Y, Kohama K: Drebrin, a development-associated brain protein from rat embryo, causes the dissociation of tropomyosin from actin filaments. J Biol Chem. 1994 Nov 25;269(47):29928-33. [Abstract]
  • *Sasaki Y, Hayashi K, Shirao T, Ishikawa R, Kohama K: Inhibition by drebrin of the actin-bundling activity of brain fascin, a protein localized in filopodia of growth cones. J Neurochem. 1996 Mar;66(3):980-8. [Abstract]
  • *Hayashi K, Ishikawa R, Ye LH, He XL, Takata K, Kohama K, Shirao T: Modulatory role of drebrin on the cytoskeleton within dendritic spines in the rat cerebral cortex. J Neurosci. 1996 Nov 15;16(22):7161-70. [Abstract] [Full Text]
  • *Hayashi K, Ishikawa R, Kawai-Hirai R, Takagi T, Taketomi A, Shirao T: Domain analysis of the actin-binding and actin-remodeling activities of drebrin. Exp Cell Res. 1999 Dec 15;253(2):673-80. [Abstract]
  • *Pérez-Martínez M, Gordón-Alonso M, Cabrero JR, Barrero-Villar M, Rey M, Mittelbrunn M, Lamana A, Morlino G, Calabia C, Yamazaki H, Shirao T, Vázquez J, González-Amaro R, Veiga E, Sánchez-Madrid F: F-actin-binding protein drebrin regulates CXCR4 recruitment to the immune synapse. J Cell Sci. 2010 Apr 1;123(Pt 7):1160-70. [Abstract] [Full Text]
  • Product Specific References

  • *Vaskova M, Kovac M, Volna P, Angelisova P, Mejstrikova E, Zuna J, Brdicka T, Hrusak O: High expression of cytoskeletal protein drebrin in TEL/AML1pos B-cell precursor acute lymphoblastic leukemia identified by a novel monoclonal antibody. Leuk Res. 2011 Aug;35(8):1111-3. Epub 2011 Apr 16. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data


    Drebrin supernatant WB


    Fig. 1. Western blotting analysis of drebrin expression in REH, Ramos, and Nalm-6 cell lysate using mouse monoclonal antibody DBN-N-03.




    Drebrin MC
    Fig. 2


    Fig. 2. Mass cytometry of brain tumor cells with anti-drebrin (DBN-N-03) 152Sm. Gated on singlets. 


    1P-694


    Fig. 3. Intracellular staining of drebrin in NALM-6 cells using anti-drebrin (DBN-N-03) PE.

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