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Mouse IgG2b Isotype Control

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Product No. Form Quantity Stock Price Datasheet Shop
  11-692-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-692-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1B-692-C025 biotin 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1B-692-C100 biotin 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-692-C025 FITC 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1F-692-C100 FITC 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1P-692-C025 PE 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1P-692-C100 PE 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1A-692-C025 APC 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1A-692-C100 APC 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  T8-692-C025 PE-Cy™5 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  T8-692-C100 PE-Cy™5 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  PC-692-C025 PerCP 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  PC-692-C100 PerCP 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  T9-692-C025 PerCP-Cy™5.5 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  T9-692-C100 PerCP-Cy™5.5 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MPC-11 was generated against an epitope irrelevant for human, mouse, and rat material, and can thus be used for evaluation of the background staining that is caused by general nonspecific interactions between an mouse IgG2b molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.

Specificity

This mouse IgG2b (kappa) monoclonal antibody (clone MPC-11) reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse, and rat tissues.

Regulatory Status

Immunogen

KLH-coupled trinitrophenol

Negative Species:

  • Human
  • Mouse
  • Rat
  • Other species Not tested

Applications:

  • Flow Cytometry
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry (paraffin sections)
  • Immunohistochemistry (frozen sections)
  • Immunocytochemistry
  • Control Experiments
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Im JS, Tapinos N, Chae GT, Illarionov PA, Besra GS, DeVries GH, Modlin RL, Sieling PA, Rambukkana A, Porcelli SA: Expression of CD1d molecules by human schwann cells and potential interactions with immunoregulatory invariant NK T cells. J Immunol. 2006 Oct 15;177(8):5226-35. [Abstract] [Full Text]
  • *Smed-S÷rensen A, Moll M, Cheng TY, LorÚ K, Norlin AC, Perbeck L, Moody DB, Spetz AL, Sandberg JK: IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa. Blood. 2008 May 15;111(10):5037-46. [Abstract] [Full Text]
  • *Gupta D, Wang Q, Vinson C, Dziarski R: Bacterial peptidoglycan induces CD14-dependent activation of transcription factors CREB/ATF and AP-1. J Biol Chem. 1999 May 14;274(20):14012-20. [Abstract] [Full Text]
  • *Dewals BG, Vanderplasschen A: Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4- T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid tissues. Vet Res. 2011 Aug 22;42(1):95. [Abstract] [Full Text]
  • *Liang XS, Zhou Y, Li CZ, Wan MB: Natural course of chronic hepatitis B is characterized by changing patterns of programmed death type-1 of CD8-positive T cells. World J Gastroenterol. 2010 Feb 7;16(5):618-24. [Abstract] [Full Text]
  • *And other.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    MPC-11 PerCP-Cy5.5


    Fig. 1. Example of nonspecific mouse IgG2b (MPC-11) PerCP-CyTM5.5 signal on human peripheral blood; surface staining, 1 μg/ml.


    MPC-11 PE-Cy5


    Fig. 2. Example of nonspecific mouse IgG2b (MPC-11) PE-CyTM5 signal on human peripheral blood; surface staining, 1 μg/ml.


    MPC-11 APC


    Fig. 3. Example of nonspecific mouse IgG2b (MPC-11) APC signal on human peripheral blood; surface staining, 1 μg/ml.



    MPC-11 biotin


    Fig. 4. Example of nonspecific mouse IgG2b (MPC-11) biotin signal on human peripheral blood; surface staining, 16 μg/ml.


    MPC-11 PE


    Fig. 5. Example of nonspecific mouse IgG2b (MPC-11) PE signal on human peripheral blood; surface staining, 3 μg/ml.



    MPC-11 PerCP


    Fig. 6. Example of nonspecific mouse IgG2b (MPC-11) PerCP signal on human peripheral blood; surface staining, 8 μg/ml.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO┤s term and conditions which are available at www.exbio.cz.
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    Cy™ and CyDye™ are registered trademarks of GE Healthcare.
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